Abstract Detail

Mycological Section

Lee, Aslan Hwanhwi [1], Kim, Jae-Jin [2].

Removal of anthracene by a new MnP gene from Peniophora incarnata KUC8836 in Saccharomyces cerevisiae.

Biotechnological applications for biodegradation of recalcitrant organic pollutants have been explored using various fungal resources throughout the world. Polycyclic aromatic hydrocarbons (PAHs), one of recalcitrant organic pollutants have intimidated environment with threats as well as human health chronically. PAHs are well known as hazardous xenobiotics and carcinogenicity only after they are activated by xenobiotic-metabolizing enzymes to strongly reactive metabolites which are capable of attacking cellular DNA and tissue. To achieve enhanced biodegradation of PAHs, ligninolytic enzyme genes secreted from fungi have been recently investigated including laccase, manganese-dependent peroxidase (MnP), and lignin peroxidase (LiP). The white rot fungus Peniophora incarnata KUC8836 has been attractive bio-resource for degradation of various PAHs. Previously, the gene encoding laccase and MnP from the fungus was newly ascertained as Pilc1 and pimp1 during degradation of pyrene and anthracene, respectively. And heterologous expression of pimp1 was carried out in Saccharomyces cerevisiae BY4741, resulting in production of MnP from the transformants via pESC-URA vector containing GAL10 promoter. In the current study, the selected transformant was investigated its enzymatic degradability of anthracene. The transformant was cultured using Erlenmeyer flasks (100 mL) containing 50 ml of indicated galactose-URA liquid medium. In addition, 30 mg L^-1 anthracene dissolved in 1 mL of acetone was added into the medium, and a total of 0.5 g L^-1 Tween 80 was supplemented to the medium for the induction of the MnP expression. And the culture was incubated at 30^oC (200 rpm) for 14 days. At the end of the incubation, degradation rate of anthracene was analyzed by GC-MS, and the corresponding MnP activity was assessed every day during incubation. In the results, anthracene was degraded by the transformant with 4.5 %, while wild type Saccharomyces cerevisiae (WT) did not demonstrate degradability. Interestingly, the transformant treated with Tween 80 showed a significant degradation rate (6.5%) of anthracene. And its MnP acivity was secreted with the highest efficiency (2.49 U mL^-1). Consequently, pimp1 might be useful for biodegradation of PAHs and gene expression technologies. To advanced bioremediation of PAHs, genetic characteristics should be more investigated using the excellent MnP gene, pimp1 and the white rot fungus, P. incarnata KUC8836.

1 - Korea University, Division of Environmental Science & Ecological Engineering, 145, Anam-ro, Seongbuk-gu, Seoul, 139-713, Republic of Korea
2 - Korea University, Division of Environmental Science & Ecological Engineering, 145, Anam-ro, Seongbuk-gu, Seoul, 136-713, Republic of Korea

Keywords:
Anthracene
Biodegradation
Peniophora incarnata
manganese-dependent peroxidase (MnP) 
Saccharomyces cerevisiae
White rot wood decay fungi.

Presentation Type: Oral Paper:Papers for Sections
Session: 12
Location: Salon 1/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 2:15 PM
Number: 12004
Abstract ID:396
Candidate for Awards:None