Abstract Detail

Systematics Section/ASPT

Wiersema, John H. [1], Borsch, Thomas [2], Hellquist, C. Barre [3], Loehne, Cornelia [4], Govers, Kim [4].

A newly discovered Central Canadian endemic water-lily, Nymphaea loriana (Nymphaeaceae).

While herbarium and field observations of morphologically intermediate water-lilies in central Canada suggested a hybrid origin involving Nymphaea odorata Aiton and N. leibergii Morong, the plants involved demonstrated a high degree of fertility. Molecular sequencing of the nrITS and the plastid rps4–trnT–trnF regions was completed, with sampling of all members of the north temperate Nymphaea subg. Nymphaea clade, as well as other naturally occurring water-lily hybrids in North America. The results indicated that individuals of N. leibergii and of N. odorata were the maternal and paternal parents, respectively. Comparable hybrids of New England have all proven to be sterile, are genetically variable, and probably are F1. However, the plants of east-central Saskatchewan and west-central Manitoba are fully fertile and genetically uniform based on ISSR and sequence data. On the basis of this evidence, the latter have been described as the new species, Nymphaea loriana, which may have originated during the Holocene climatic optimum about 6000 years ago in a past contact zone of the parents. Other hybrids between N. odorata and N. tetragona Georgi, as well as between N. leibergii and N. tetragona, were always sterile. Gene trees of the temperate clade of Nymphaea converge on a clade of small-flowered water-lilies (sect. Chamaenymphaea), including N. leibergii, N. tetragona, and N. pygmaea, while nuclear ITS resolves an American subclade (N. mexicana Zucc. – N. odorata) sister to all remaining temperate species. This split into two major subclades also appears in the less resolved rps4–trnT–trnF tree. Thus N. loriana resulted from a reticulation between long-separated parental lineages.

1 - 525 Watts Avenue, Gambrills, MD, 21054, USA
2 - Freie Universitaet Berlin, Botanischer Garten & Museum, Konigin Luise-Str. 6-8, Berlin, N/A, 14195, Germany
3 - Massachusetts College of Liberal Arts, Department of Biology, North Adams, MA, 01247-4100, USA
4 - Botanischer Garten und Botanisches Museum Berlin-Dahlem and Institut f, Dahlem Centre of Plant Sciences, Freie Universität Berlin, Königin-Luise-Str. 6-8, Berlin, 14195, Germany
5 - Botanischer Garten und Botanisches Museum Berlin-Dahlem and Institut f, Dahlem Centre of Plant Sciences, Freie Universität Berlin, Königin-Luise-Str. 6-8, Berlin, 14195, Germany

Keywords:
Phylogeny
hybridization.

Presentation Type: Oral Paper:Papers for Sections
Session: 33
Location: Salon 10/The Shaw Conference Centre
Date: Tuesday, July 28th, 2015
Time: 10:45 AM
Number: 33011
Abstract ID:645
Candidate for Awards:None