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Abstract Detail



Mycological Section

Krizsan, Krisztina [1], Almasi, Eva [1], Kohler, Annegret [2], Martin, Francis [2], Hibbett, David [4], Nagy, Laszlo [1].

Behind the scenes: a transcriptomic study of the genomic processes underlying the evolution of complex fruiting body morphologies in Agaricomycetes.

The genomic changes underlying the evolution of complex multicellular structures of fungi are hardly known. The most typical manifestations of complex multicellularity in fungi are fruiting bodies, 3-dimensional structures involved in sexual spore production. Fruiting bodies evolved in most major clades, with their highest complexity probably found in the Agaricomycetes. Here, we set out to identify gene families responsible for morphogenetic changes during fruiting body formation by using transcriptomic and comparative genomic approaches. We determined the transcriptome profile of different developmental stages and tissue types (cap, lamellae, stipe) of the mushroom species Lentinus tigrinus strain RLG-9953-Sp (Polyporales, Agaricomycetes). We analysed gene expression levels in vegetative mycelia, hyphal knots, stage I and stage II primordia as well as young and mature fruiting bodies. The transcriptome profiles at various stages and different structures of L. tigrinus are compared with that of related species such as Agaricus bisporus, Coprinopsis cinerea and Schizophyllum commune. Comparisons with published transcriptome data revealed conserved elements of fungal fruiting body development, including several gene families which members were upregulated in many or all of the compared species. These appear to be primarily involved in biosynthetic processes. Comparative phylogenomic analyses of the detected gene families revealed extensive diversification within mushroom-forming fungi and the fate of development-related gene families in species that do not produce complex fruiting bodies. Our work represents a first step towards identifying the basic genomic processes involved in the evolution of complex fruiting body structures in fungi.


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1 - HAS BRC, Synthetic and Systems Biology Unit, Institute of Biochemistry, Temesvari krt 62, Szeged, 6726, Hungary
2 - Laboratory of Excellence Advanced Research on the Biology of Tree and , Institut National de la Recherche Agronomique (INRA) , Champenoux, 1136, France
3 - Laboratory of Excellence Advanced Research on the Biology of Tree and , Institut National de la Recherche Agronomique (INRA) , Champenoux, 1136, France
4 - Clark University, Biology Department, Worcester, MA, 01610, USA

Keywords:
evo devo
multicellularity
Agaricomycetes
fungal development
whole-genome and transcriptome data.

Presentation Type: Poster:Posters for Sections
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PMY004
Abstract ID:1001
Candidate for Awards:None


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