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Abstract Detail



What can Next Generation Fungal Genome Sequencing do for you?

Peng, You Liang [1], Yin, Chang Fa [1], Zhao, Wen Sheng [1], Yang, Jun [1], Liu, Cui Cui [1], Fang, Wen Wen [1], Zhou, Si [1].

Comparative genomics of the rice blast fungus Magnaporthe oryzae.

Magnaporthe oryzae causes the rice blast disease, which is one of the most destructive diseases on cultivated rice. The fungal pathogen populations are rich in diversity of pathotypes and variation of other phenotypes. In a previous study, we did whole genome sequencing of two field isolates of M. oryzae and made a comparative analysis on their difference from the sequenced laboratory strain 70-15. The comparison showed that the isolates have a similar genome size but are significantly different in a number of gene families, isolate-specific genes and loci of transposon-like elements. In particular, we found that transposon-like elements tend to be associated with isolate-specific or duplicated sequences. To better understand genetic variations of M. oryzae in nature, we further sequenced the genome of more than 160 field isolates, which were collected from 6 different rice growing counties in southern and northern China and were different from each other in pathotypes. We have assembled the genome sequences of each isolates and confirmed that more than 97% of the genome sequence from 70-15 was covered in our assembled sequences of the isolates. Further comparison showed that the isolates from southern China are phylogenetically different from those from northern China. We are now identifying M. oryzae-specific genes that are identical and variable among isolates, respectively. We will also try to identify genes that are related with pathotype differentiation.


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1 - China Agricultural University, Department of Plant Pathology, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, P.R.China, Beijing, 100193, China

Keywords:
effector
pathotype
genetic variation.

Presentation Type: Symposium Presentation
Session: SY09
Location: Salon 3/The Shaw Conference Centre
Date: Tuesday, July 28th, 2015
Time: 10:45 AM
Number: SY09007
Abstract ID:1009
Candidate for Awards:None


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