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Abstract Detail

Biological Control of diseases and weeds (CFBC)

Hannan, Abdul [1], Mukhtar, irum [2], Riaz, Kashif [3].

Diversity in Trichoderma harzianum Strains for Antagonistic Potential against major Soil Borne Plant Pathogens and its Mass Culturing.

Biofertilizers are considered important in green agriculture whereas Trichoderma harzianum has a novel superiority. It can enhance soil fertility as well as suppress soil borne plant Pathogens (SBPPs). Current research is an effort to exploit actual potential of local strains of T. harzianum to manage SBPP. The study was aimed to introduce a dual action biofertilizer for vegetable growers and specifically for kitchen gardeners. During 2014, a study was conducted and diversity of T. harzianum was determined in Faisalabad Division, Punjab, Pakistan. Eighty four strains of T. harzianum were identified and placed in repository of First fungal culture Bank of Pakistan (FFCBP). Different types of assays revealed growth inhibition with different degree of success variations efficient strains significantly (P<0.05) with 72% of reduction in colony diameter of SBPP. These strains were categorized on the bases of antagonistic efficiency against two major SBPPs i.e. Rhizoctonia solani and Fusarium oxysporum. The multi directional approach concluded that efficient producers are fast growing (0.375mm/h) with color light shade. The data revealed that the strains of T. harzianum isolated from naturally diseased suppressed areas showed higher percentage of reduction in colony diameter over control (68.8 to 78.2%) during dual culture test against SBPPs. Moreover, the results showed a detrimental interaction between population of SBPPs and antagonistic potential of biocontrol agent with positive correlation. The studies were further extended to characterization and cultivation of T. harzianum on different substrates where the strains with higher pathocidal prospective were characterized for maximum yield of conidia on cheaper substrates under solid-state fermentation. All substrates showed dissimilar response but conidial count was maximal in rice bran supplemented with chickpea flour (2.5 x 108 cfu/g) after 18 days of incubation at 27ΒΊC. The characterized strains were tested in the field where reduction in disease incidence over control was observed (52.4 to 68.0%) over control. These findings explored a possibility of cost effective technology for production of T. harzianum at pilot scale. The current studies are still in progress, where different formulation of substrates are being tested and large scale production of selected and characterized strains is under the process of optimization. Finally a standard bioassay will be conducted for quality control and product will be patented and registered.

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1 - Ghazi University D.G. Khan, Botany, Faculty of Agricultural Sciences, D G Khan, Punjab, 32200, Pakistan
2 - Division of Sciences and Technology, Education University Lahore, Lahore, Punjab, 54000, Pakistan
3 - Department of Plant Pathology, Faculty of Agriculture, University of Agriculture Faisalabad, Faisalabad , Punjab, 38000, Pakistan


Presentation Type: Oral Paper:Papers for Topics
Session: 75
Location: Salon 2/The Shaw Conference Centre
Date: Wednesday, July 29th, 2015
Time: 4:45 PM
Number: 75005
Abstract ID:1023
Candidate for Awards:None

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