Abstract Detail

Mycological Section

Almatruk, Maa [2], Hesse, Cedar H. [1], Kuske, Cheryl R. [1], Porras-Alfaro, Andrea [2].

Yeast diversity in North Carolina Duke Forest.

Yeasts have a major impact on human, plant, and animal health. However, little is known about their diversity, ecology, and distribution in natural ecosystems. The objective of this research was to explore yeast diversity and distribution in different soil horizons in the Duke Forest, NC. Soils samples were diluted and yeast colonies were isolated in peptone-yeast-glucose (PYG) and Malt Extract Agar (MEA) media at 25°C and 35 °C. A total of 66 pure cultures were obtained, extracted, and sequenced using the ITS, LSU, and SSU rRNA regions. Sixty-five percent of yeast isolates belong to the Basidiomycota and 35% belong to Ascomycota. Sporidioboales and Saccharomycetales were the dominant orders and the most abundant taxa were represented by Rhodotorula, Cryptococcus, Trichosporon, Sporidiobolus, and Saccharomyces. Yeast distribution on different soil horizons will be compared to Illumina data obtained from the same soils.

1 - Los Alamos National Laboratory, Bioscience Division, Los Alamos, NM, 87544, USA
2 - Western Illinois University, Biological Sciences, 1 University Circle, Macomb, IL, 61455, USA

Keywords:
yeast
Sporidioboales
Saccharomycetales
diversity.

Presentation Type: Poster:Posters for Sections
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PMY054
Abstract ID:1024
Candidate for Awards:MSA Best Poster Presentation Award by a Graduate Student