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Abstract Detail

Host/Plant Pathogen Interactions and Plant Health Management

Anctil, Félicia [1], Joly, David L. [1].

Functional approaches for the characterization of effectors in Phytophthora infestans.

Phytophthora infestans (Mont.) De Bary is the oomycete responsible for potato late blight. This phytopathogenic agent relies on its capacity to secrete effector proteins to modulate plant immunity and allow parasitic colonization. Some of these effectors may be recognized by plant resistance proteins and triggers a defensive reaction known as the hypersensitive response. In this case, these effectors are called avirulence factors. In susceptible plants, these effectors appears to contribute to virulence. With the advent of new sequencing technologies, identification of candidate effectors can be done rapidly, and their functional characterization remains the main bottleneck. Various approaches have been developed toward this goal over the last decade. Here, the use of a heterologous bacterial system where the expression of an effector in a first bacteria (Pseudomonas fluorescens “EtHAn”) enable the infection by a second bacteria (Pseudomonas syringae pv. tomato) in Nicotiana benthamiana highlights the capacity of some of these effectors to supress plant immune responses. This will lead to a better understanding of virulence mechanisms in pathogens and of immunity-related processes in plants.

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1 - Université de Moncton, Département de biologie, 18 avenue Antonine-Maillet, Moncton, NB, E1A 3E9, Canada

Heterologous system
Plant-Pathogen Interaction
Phytophthora infestans
Pseudomonas syringae.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PPA016
Abstract ID:1037
Candidate for Awards:None

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