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Abstract Detail

Biochemistry, metabolism, carbon flux

Arif, Muhammad [1], Riaz, Muhammad [2], Pauls, K. Peter [2].

Measurement of neutral detergent fibre, acid detergent fibre and acid detergent lignin in corn stalks and cobs fibres by Fourier-transform infrared spectroscopy.

This study was initiated to investigate the potential of using partial least squares (PLS) and Fourier-transform infrared (FTIR) spectroscopy to predict neutral detergent fibre (NDF), acid detergent fibre (ADF) and acid detergent lignin (ADL) parameters in corn stalk and cobs fibres as alternative procedure to wet chemistry. Forty recombinant inbred lines (RILs) from a CG62 x CO387 cross were grown in replicated plots (3) in four environments in Ontario. The dried corn stalks and cobs were ground in Wiley mill using 2 mm sieve. NDF, ADF and ADL were measured in 96 corn stalk and cobs fibre samples by ANKOM sequential analysis to develop the PLS predictive model. Infrared spectra were collected from 504 dried ground samples (in duplicate) by attenuated total reflectance (ATR) from 400 to 4000 cm-1 using a FTIR spectrometer. All spectra were averaged over 64 scans at a resolution of 4 cm-1, derivatized to the first Savitsky-Golay derivative (15 units of filter width and polynomial order of 2), normalized and mean centre scaled. A PLS statistical procedure was used to predict dependent variables from a set of independent variables. The predicted NDF, ADF and ADL values were positively and highly significantly correlated with the measured values (R2 of NDF, ADF and ADL 0.94, 0.89 and 0.67, respectively). The corn stalk and cobs RILs showed significant variations for NDF, ADF and ADL contents in their fibres. This study demonstrated that FTIR spectroscopy as a cost effective and high through-put alternative to wet chemistry for compositional analysis in a large set of agricultural feed stocks.

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1 - University of Guelph, Plant Agriculture, 50 Stone Raod East, Guelph, ON, N1G2W1, Canada
2 - University of Guelph, Plant Agriculture, 50 Stone Road East, Guelph, ON, N1G2W1, Canada


Presentation Type: Poster:Posters for Topics
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PBC008
Abstract ID:1046
Candidate for Awards:None

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