| Abstract Detail
Biochemistry, metabolism, carbon flux Wang, li [1], Xue, donghua [2], Gao, xiang [1], Wei, Sun [3], Meng, xiangyu [1], Liang, lingjie [1]. Molecular and Biochemical Analysis of Chalcone Synthase from Freesia hybrida in Flavonoid Biosynthetic Pathway. Flavonoids are one of the most important plant-specific secondary metabolites, and they possess significant and diverse biological functions, including protection against UV radiation, defense against phytopathogens and herbivores, pollen growth and development, and, importantly, they are major pigments for flowers, fruits, seeds, and leaves. Meanwhile, it has been reported that flavonoids also have a close connection with human health. Chalcone synthase(CHS) is the first key enzyme in the biosynthesis of flavonoid which catalyzes the formation of naringenin chalcone. Because of the essential role of CHS in flavonoid biosynthesis, a cDNA encoding the CHS from Freesia hybrida(FhCHS1) was successfully isolated, and analyses of its amino acid sequences and crystal structure reveal that FhCHS1 may have the typical CHS enzymatic function. Indeed, the subsequent enzymatic assays indicate that FhCHS1 can catalyze the condensation of p-coumaroyl-CoA with malonyl-CoA to produce naringenin chalcone. Furthermore, we show that expression of FhCHS1 in flowers is developmentally regulated in a way that associates with their anthocyanin pigmentation, and expression profile in non-pigmented organs demonstrates that FhCHS1 is not flower specific and can be involved in the biosynthesis of other flavonoids. Finally, we report that FhCHS1 can fully restore the pigmentation phenotype of Arabidopsis tt4 mutants, and alter the flower color of petunia from white to pink with the change of flavonoid. To the best of our knowledge, this is the first study to identify the characterization of CHS from Freesia hybrid, and our findings also have significant implications in modifying coloration in other important floricultural plants and/or improving nutritive values in plant species of interest.
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1 - Northeast Normal University, Institute of Genetics and Cytology, 5268 Renmin Street, changchun, jilin, 130024, China
2 - Changchun University of Technology, School of Chemistry and Life Sciences, 2055 Yanan Road, changchun, jilin, 130012, China
3 - Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province, China
Keywords:
Freesia hybrida
flavonoid
chalcone synthase
A. thaliana tt4 mutants
transgenic petunia.
Presentation Type: Poster:Posters for Topics
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PBC005
Abstract ID:105
Candidate for Awards:None |
