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Abstract Detail



Biotic and abiotic stress

Souter, Jodi R. [1], Todd, Christopher D. [1].

The Role of Ureides during Abiotic Stress.

Ureides are nitrogen-containing molecules formed during the oxidation of purines. These molecules, specifically uric acid, allantoin and allantoate, accumulate during abiotic stress. Ureides have been hypothesized to function as Reactive Oxygen Species (ROS) scavengers in plants during abiotic stress but a closer look at the compartmentalization and the antioxidant function of these molecules raises questions associated with this role. Ureides accumulate in soybean leaves when plants are subjected to drought stress and in response to methyl viologen (MV), a ROS generator. In this study, we sought to determine whether exogenous ureides, specifically uric acid and allantoin, would decrease the amount of ROS produced by the leaf and protect the leaf from oxidative stress. We quantified ureide levels, hydrogen peroxide (H2O2) and superoxide (O2-), and performed electrolyte leakage assays to observe plant response to MV and H2O2 with and without exogenously supplied uric acid and allantoin. No decrease in ureide amount or ROS amount was seen in solution in the absence of leaf tissue. However, when leaves were present, pretreatment with allantoin reduced the amount of exogenously applied H2O2 that leaves were floated in. Electrolyte leakage assays showed that pretreatment with uric acid decreased cell death following H2O2 and MV treatment. These results suggests instead of ureides directly scavenging ROS, these molecules may have an indirect role in the scavenging of ROS and survival of plant cells during periods of oxidative stress.


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1 - University of Saskatchewan, Department of Biology, 112 Science Place, W.P.Thompson Bld, Saskatoon, SK, S7N 5E2, Canada

Keywords:
Ureide
Abiotic stress
reactive oxygen species
Allantoin
Uric Acid.

Presentation Type: Oral Paper:Papers for Topics
Session: 20
Location: Salon 13/14/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 2:15 PM
Number: 20003
Abstract ID:1123
Candidate for Awards:CSPB President's Award for Best Student Presentation


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