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Abstract Detail



Genomics / Proteomics

Nepal, Madhav P. [1], Andersen, Ethan [2], Shaw, Samantha R. [2], Gonzalez, Jose L. [2], Yen, Yang [1], Reese, R. Neil [1].

Comparative Genomics of CNL Disease Resistance Genes in Six Grass Species.

Plant disease resistance genes (R-genes) encode proteins involved in plant defense against pathogens. The main objectives of this study were to 1) identify CNL (Coiled-coil, Nucleotide-binding site, and Leucine-rich repeat) R-genes in five grass species (Aegilops tauschii, barley, foxtail millet, rice, and wheat), and 2) elucidate their evolutionary relationships. We performed Hidden Markov Model (HMM) search for the CNL genes in each of the five genomes. Genes were identified using conserved motifs within the NB-ARC region, namely the P-Loop, Kinase2, and GLPL motifs. Phylogenetic analyses were performed using Maximum Likelihood method. The Aegilops tauschii genome was closely compared to wheat, since it is the likely contributor of the wheat D genome. We identified 402, 175, 237, 149, and 617 CNL genes in Aegilops tauschii, barley, Foxtail Millet, rice, and wheat, respectively. Out of the four clades (A-D) previously reported in dicot genomes, our preliminary data showed that the grass genomes have retained clades B and C, with severe reduction in clade A and complete loss of clade D. Interesting patterns of gene duplication and Ks values will be discussed in our presentation.


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Related Links:
SDSU Nepal Lab


1 - South Dakota State University, Biology And Microbiology, Northern Plain Biostress Laboratory, Brookings, SD, 57007, USA
2 - South Dakota State University, Department of Biology/Microbiology, Brookings, SD, 57007, United States

Keywords:
CNL Genes
Disease Resistance
NB-ARC
Grass Genomes
NBS-LRR
gene duplication
Ks Values.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:45 PM
Number: PGP012
Abstract ID:1174
Candidate for Awards:None


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