| Abstract Detail
Genomics / Proteomics Nepal, Madhav P. [1], Andersen, Ethan [2], Shaw, Samantha R. [2], Gonzalez, Jose L. [2], Yen, Yang [1], Reese, R. Neil [1]. Comparative Genomics of CNL Disease Resistance Genes in Six Grass Species. Plant disease resistance genes (R-genes) encode proteins involved in plant defense against pathogens. The main objectives of this study were to 1) identify CNL (Coiled-coil, Nucleotide-binding site, and Leucine-rich repeat) R-genes in five grass species (Aegilops tauschii, barley, foxtail millet, rice, and wheat), and 2) elucidate their evolutionary relationships. We performed Hidden Markov Model (HMM) search for the CNL genes in each of the five genomes. Genes were identified using conserved motifs within the NB-ARC region, namely the P-Loop, Kinase2, and GLPL motifs. Phylogenetic analyses were performed using Maximum Likelihood method. The Aegilops tauschii genome was closely compared to wheat, since it is the likely contributor of the wheat D genome. We identified 402, 175, 237, 149, and 617 CNL genes in Aegilops tauschii, barley, Foxtail Millet, rice, and wheat, respectively. Out of the four clades (A-D) previously reported in dicot genomes, our preliminary data showed that the grass genomes have retained clades B and C, with severe reduction in clade A and complete loss of clade D. Interesting patterns of gene duplication and Ks values will be discussed in our presentation. Log in to add this item to your schedule
Related Links: SDSU Nepal Lab
1 - South Dakota State University, Biology And Microbiology, Northern Plain Biostress Laboratory, Brookings, SD, 57007, USA 2 - South Dakota State University, Department of Biology/Microbiology, Brookings, SD, 57007, United States
Keywords: CNL Genes Disease Resistance NB-ARC Grass Genomes NBS-LRR gene duplication Ks Values.
Presentation Type: Poster:Posters for Topics Session: P Location: Hall D/The Shaw Conference Centre Date: Monday, July 27th, 2015 Time: 5:45 PM Number: PGP012 Abstract ID:1174 Candidate for Awards:None |