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Abstract Detail

Genetics Section

Zantinge, Jennifer [1], Juskiw, Patricia [2], Xue, Shiming [1], Xi, Kequan [3], Holtz, Michael [1], Helm, Jim [1].

Pyramiding by marker assisted selection resistance to Scald into malting barley.

Scald (Rhynchosporium commune) of barley (Hordeum vulgare) is a prevalent disease in central Alberta, Canada and causes considerable yield and quality losses. Scald can rapidly change in pathotype composition and frequency, thereby making it difficult to develop durable resistance in barley. Previous studies have shown that the cultivar ‘Seebe’ carries durable genetic resistance; however, breeders have found this resistance difficult to transfer into new malting barley types. Over the past decade we have identified multiple quantitative trait loci (QTLs) linked to resistance to R. commune from ‘Seebe’ and other resistant cultivars utilized in the 2-row malting barley breeding program at the Field Crop Development Centre, Lacombe, Alberta. The objective of this paper is to determine the effectiveness of marker selection for scald over the past 5 years. Our lab has routinely genotyped approximately 3500 breeding lines yearly, with markers linked to scald resistance QTLs. Simple sequence repeat (SSR) markers were used to genotype several major QTLs on chromosomes 2H, 3H(Rrs1-3,4 complex), 6H(Rrs13) and 7H(Rrs15, Rrs2); (Minor QTLs) on 4H and 5H. In those populations where polymorphisms were present, leaf samples were collected at the 3-5 leaf stage from plants grown in the field at Lacombe. DNA was extracted, specific SSR primers were used to amplify DNA, and fragments were separated by gel electrophoresis. A predictive rating for scald resistance was given to each line based on the presence or absence of these QTLs. A susceptible allele on the 4H chromosome from the malt cultivar ‘Harrington’ was removed from breeding populations in the first 2 years of selection. We then continued to select for loci on 3H, 5H, and 7H. Our selection using molecular markers for scald resistance has been successful at breaking the negative linkage to malt quality while improving scald resistance. However, the effectiveness of our selection for scald resistance varies depending on the population and the genes present within the population. As well as, the levels of scald resistance presented in lines varied from year to year due to the field environment and the scald races present in the field.

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1 - Field Crop Development Center, Alberta Agriculture and Rural Development, 5030 - 50 Street, Lacombe, AB, T4L1W8, Canada
2 - Field Crop Development Centre, Alberta Agriculture and Rural Development, 5030 50th Street, Lacombe, AB, T4L 1W8, Canada
3 - Alberta Agriculture, Field Crop Development Centre, 6000 C and E Trail, Lacombe, AB, T4L 1W1, Canada

Molecular markers.

Presentation Type: Poster:Posters for Sections
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PGN016
Abstract ID:1225
Candidate for Awards:None

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