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Abstract Detail



Population Genetics

Lim, Chae Eun [1], Choi, Ji-Eun [1], Yu, Jeong-Nam [2], Lee, Byoung-Yoon [1].

Population genetic variability and structure of Aconitum austrokoreense (Ranunculaceae) endemic to Korean peninsula inferred from microsatellite markers and chloroplast intergenic spacer marker.

Aconitum austrokoreense Koidz. An endemic herbal species with medicinal values, is narrowly distributed in a few locations in rocky habitats of southeast of Korean peninsula. Its natural populations are declining in their habitats due to very rare distribution, over-harvesting for traditional medicinal use, and loss of habitat. Thus, A. austrokoreense was listed in the Red Data Book of Endangered Vascular Plants in Korea. In this study, we have developed 5 new microsatellite markers in A. austrokoreense, a rare endemic species in Korea, using a next-generation sequencing. All novel microsatellite markers were successfully genotyped using 300 samples from seven populations of Gyeongsang and Jeolla Province in Korea. The number of alleles ranged from 2 to 7 per locus in each population. Observed and expected heterozygosities ranged from 0.031 to 0.938 and from 0.031 to 0.697, respectively. These markers will be valuable tools for assessing the genetic diversity of A. austrokoreense and for germplasm conservation of this endangered species. In addition, two genetic clusters were detected by the Structure analysis based on the microsatellite markers and chloroplast intergenic spacer marker.


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1 - National Institute of Biological Resources, Plant Resource Division, Environmental Research Complex, Hwangyeong-ro 42, Seo-gu, Incheon, 404-708, Korea
2 - National Institute of Biological Resources, Biological and Genetic Resources Assessment Division, Environmental Research Complex, Hwangyeong-ro 42, Seo-gu, Incheon, 404-708, Korea

Keywords:
Aconitum austrokoreense
Molecular markers
Medicinal plants
endemic
rare plant.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PPG008
Abstract ID:1253
Candidate for Awards:None


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