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Abstract Detail



Genetics Section

Mohamed, Abeer [1], Timothy, Short [2].

New tools to study phytochromes in fern Ceratopteris.

The lifecycle of the fern Ceratopteris richardii comprises of simple free-living gametophyte and complex vascular sporophyte stages that are capable of growing independently of one another. This allows the study of various phenomena at both stages. Consequently, both cellular and whole plant studies can be carried out simultaneously in a genetically natural and stable system. Although the photobiology of ferns is well characterized at the physiological level, little is known at the molecular level. Molecular studies of photoreceptors in Ceratopteris is not well advanced compared to moss and Arabidopsis due to difficulty of establishing reliable stable or transient transformation system. Using the microinjection technique we were capable of studying localization of many GFP-fused phytochromes under different light regimes in Ceratopteris prothalli. Using virus induced gene silencing (VIGS), we are optimizing a method to knockdown phytochromes in Ceratopteris richardii. Tobacco Rattle Virus TRV1 and TRV2, the two components of the VIGS system were used to facilitate downregulation of target gene. Both transient transformation techniques should contribute to expanding transformation with gene silencing constructs, gene or promoter trapping constructs and many other genetic tools to advance experimentation on this unique fern.


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1 - Faculty of Agriculture (Saba Basha), Alexandria Univ. , Agricultural Botany and Genetics Dept., 22 Tag El-Roasaa St., Saba Basha, Alexandria, Egypt
2 - Queens College, CUNY, Biology, 65-30 Kissena Blvd, Flushing, NY, 11367, USA

Keywords:
Ceratopteris richardii
phytochrome
microinjection
RNAi.

Presentation Type: Poster:Posters for Sections
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PGN017
Abstract ID:1267
Candidate for Awards:None


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