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Abstract Detail

Genomics / Proteomics

Joshi, Raj Kumar [1], Megha, Swati [2], Rahman, Muhammad Hafiz [2], Basu, Urmila [3], Kav, Nat N. V. [4].

Dual RNA-Seq transcriptome profiling of canola (Brassica napus L.) and Sclerotinia sclerotiorum during a compatible interaction.

Brassica napus (canola) is one of the most important oilseed crops being cultivated worldwide and is the second largest crop grown in Canada. Productivity of canola can be negatively affected by Sclerotinia stem rot, caused by the necrotrophic, non-host specific fungal phytopathogen, Sclerotinia sclerotiorum. Sclerotinia stem rot outbreaks cause substantial yield losses worldwide including in North America. Insights into the molecular processes accompanying the interaction of S. sclerotiorum with B. napus may be very useful to understand the role of specific genes during pathogenesis of this disease. In the past, most of gene expression studies have studied the molecular events which occur during host-pathogen interaction separately. In this study, the transcriptomes of canola and S. sclerotiorum were analyzed simultaneously during a compatible interaction at 24 and 48 hours post inoculation (hpi) using the stranded dual RNA-Seq technique. From transcriptomic data 151,460 genes from B. napus, were found to be expressed at both the time points and, among those, 80,844 genes were novel transcripts while, 22,795 genes were alternatively spliced. In the case of S. sclerotiorum, 8,058 and 11,193 genes were expressed at 24hpi and 48hpi, respectively. In addition, in the case of B. napus, 260 genes were found to be differentially expressed at both the infection stages. On one hand, genes encoding glucanases, chitinases, peroxidases, transcription factors (NAC, WRKY, EREBF) and those associated with jasmonate and ethylene signalling were found to be rapidly induced at 24hpi, in B. napus, suggesting an early adaptation of molecular processes by the host in response to the pathogen. On the other hand, cell wall degrading Carbohydrate-Active enzymes (CAZymes), MAP kinase smk1, transcription factors (C2H2 zinc finger, bZIP) and apoptotic enzymes, were among the differentially expressed genes in S. sclerotiorum, indicative of the pathogen possibly resorting to multiple strategies to induce pathogenicity in canola. Our results are presented and will be discussed within the context of the roles of the genes identified in this study during the pathogenesis of stem rot disease in canola.

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1 - Siksha 'O' Anusandhan University, Centre of Biotechnology School of Pharmaceutical Sciences , At-Kalinga Nagar, post- Ghatikia, Bhubaneswa, Odisha, 751003, India
2 - University of Alberta, Agriculture, Food and Nutritional Science, 4-10 Agriculture /Forestry Centre , Edmonton, Alberta, T6G 2P5, Canada
3 - University of Alberta, Agriculture, Food and Nutritional Science, 4-32A Ag/Forestry, Edmonton, Alberta, T6G 2P5, Canada
4 - University of Alberta, Agriculture, Food and Nutritional Science, 2-06C Agriculture/Forestry Ctr, Edmonton, Alberta, T6G 2P5, Canada

Sclerotinia sclerotiorum

Presentation Type: Poster:Posters for Topics
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:45 PM
Number: PGP016
Abstract ID:1321
Candidate for Awards:CSPB President's Award for Best Student Presentation

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