Abstract Detail

Host/Plant Pathogen Interactions and Plant Health Management

Holtz, Michael D. [1], Zantinge, Jennifer [2], Xi, Kequan [1], Turkington, T. Kelly [3].

Genetic diversity and necrotrophic effectors in the barley pathogen Rhynchosporium commune in Alberta, Canada.

Scald is an important foliar disease of barley, caused by the fungus Rhynchosporium commune Zaffarano et al. (formerly R. secalis (Oudem.) J.J. Davis). Little is known about the molecular population biology of the species within Canada. The presence of necrotrophic effectors (NEs) and the genetic diversity was determined for 230 R. commune isolates collected from barley in central Alberta. The genes for the three known NEs in the species, NIP1, NIP2, and NIP3, were present in almost all isolates studied. Genetic diversity was examined using 8 simple sequence repeat (SSR) markers. Thirty-six alleles were detected, 3 to 8 per locus. The clonal fraction among the isolates was 39%, with clones commonly being found at the same location. Linkage disequilibrium between loci was found to be insignificant. The presence of a large clonal fraction, but insignificant linkage disequilibrium indicates the species likely has a mixed reproductive system. There was little differentiation between populations. After clone correction only a small (10%) but significant differentiation (Φpt=0.095, P = 0.0001) could be detected between populations. No alleles were confined to a specific population. Moreover, cluster analysis using shared allele distances between isolates showed no differentiation between regions within central Alberta.

1 - Alberta Agriculture and Rural Development, Field Crop Development Centre, 6000 C and E Trail, Lacombe, AB, T4L 1W1, Canada
2 - Field Crop Development Center, Alberta Agriculture and Rural Development, 5030 - 50 Street, Lacombe, AB, T4L1W8, Canada
3 - Agriculture and Agri-Food Canada, Lacombe Research Centre, 6000 C and E Trail, Lacombe, AB, T4L 1W1, Canada

Keywords:
Rhynchosporium commune
scald
barley
SSRs.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PPA011
Abstract ID:1327
Candidate for Awards:None