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Abstract Detail

Genetics Section

Tack, David [1], Adams, Keith [1].

Reshaped patterns of alternative splicing after allopolyploidy in Brassica napus.

In allopolyploids, when two genomes are amalgamated into a common nucleus, the native cis-architectures of genes are placed in a new hybrid and polyploid trans-environment, releasing novel expression profiles with respect to parental patterns. In conjunction with changes in transcript levels, transcript splicing patterns undergo changes to create novel forms. We studied the degree to which alternative splicing patterns in allopolyploids differ from the parental splicing patterns and the degree to which they are reciprocated between individual instances of allopolyploidy. Using RNA-seq, we analyzed the transcriptomes of four allotetraploid Brassica napus lines and from the B. rapa and B. oleracea parental species. The analyses revealed myriad qualitative and quantitative differences in alternative splicing events among the genotypes. Several thousand alternatively spliced transcript forms were lost or gained in the polyploids, with some variation in splicing patterns among lines. Losses of alternative splicing events were more common than gains. We show that allopolyplody generates a variety of splicing landscapes from the same genetic merger, revealing both plastic and static changes in splicing, and our results complement studies of gene expression levels and patterns in allopolyploids. Our results show that changes in splicing patterns are a common occurrence after allopolyploidy and they contribute to the “transcriptome shock” experienced by newly formed allopolyploids.

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1 - University of British Columbia, Botany, 6270 University Blvd, Vancouver, BC, V6T1Z4, Canada

gene duplication
gene expression
Transcriptome profiles

Presentation Type: Oral Paper:Papers for Sections
Session: 6
Location: Salon 15/16/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 9:30 AM
Number: 6007
Abstract ID:1330
Candidate for Awards:Margaret Menzel Award

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