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Abstract Detail

Recent advances in phylogeny and systematics of Brassicaceae 

Mandáková, Terezie [1], Zozomová-Lihová, Judita [2], Lysak, Martin [3], Marhold, Karol [4].

The origin and genome structure of the European Cardamine flexuosa and related Eastern Asian allopolyploids.

The polyploid origin of the European Cardamine flexuosa and its Eastern Asian relatives was addressed using genomic in situ hybridization (GISH) and comparative chromosome painting (CCP). Our aim was to identify parental species, analyse and compare structure of parental and allopolyploid genomes, and to describe inter-genomic structural variations. The European C. flexuosa is an allotetraploid (2n = 4x = 32), originating from two diploid species, C. amara and C. hirsuta (2n = 2x = 16). A comparative cytomolecular map was established for the European C. flexuosa by CCP/GISH. The two parental species display almost perfectly conserved chromosomal collinearity for seven out of the eight chromosomes. A 13-Mb pericentric inversion distinguishes chromosome CA1 from CH1. We found one reciprocal translocation between two homeologous chromosomes in C. flexuosa. CCP/GISH uncovered the identity and structure of parental genomes in several tetra- and octoploid (2n = 8x = 64) Asian Cardamine species (C. dentipetala, C. kokaiensis, C. fallax, C. occulta, and C. scutata). GISH data suggest that all the analyzed species are allopolyploids formed via hybridization between two diploid species - C. amara and C. parviflora (or their predecessors). Species-specific chromosome rearrangements identified in both parental genomes as well as in all the allopolyploid genomes provide additional strong evidence on the parentage of C. amara and C. parviflora.

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1 - Central European Institute of Technology (CEITEC), Masaryk University, Research group Plant Cytogenomics, Kamenice 5, building A26, Brno, CZ-62500 , Czech Republic
2 - Institute of Botany, Slovak Academy of Sciences, Dubravska cesta 9, Bratislava, SK-84523, Slovakia
3 - CEITEC, Masaryk University, Kamenice 5, Brno, 62500, Czech Republic
4 - Institute Of Botany/Slovak Acad Sci, Dubravska Cesta 9, Bratislava, N/A, SK-845 23, Slovakia, +421 2 59426128

genomic in situ hybridization
comparative chromosome painting.

Presentation Type: Colloquium Presentations
Session: C2
Location: Salon 8/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 4:45 PM
Number: C2008
Abstract ID:1350
Candidate for Awards:None

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