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Abstract Detail



Genomics / Proteomics

Panes, Vivian [1].

Unraveling the Transcriptome of the Moringa oleifera Lam. Mature Embryo Using Direct RNA-Sequencing.

Moringa oleifera seeds are capable of producing 40% edible oils that are gaining importance due to its nutritional benefits. Many studies have examined M. oleifera seed oil, however these studies focused on the extraction of oil and methods of biodiesel production. There is a paucity of information on transcriptome level studies to determine the unigenes involved in oil biosynthesis metabolic pathways. The main objective of this study is to explore the transcriptome of the mature embryo of Moringa oleifera Lam. particularly the key genes related to oil biosynthesis. The transcriptome reflects the set of genes that are actively expressed at any given time produced in one or a population of cells in a given organism. Total RNA was extracted from thirty mature seed embryos obtained from 10 trees in Muñoz, Nueva Ecija. RNA were pooled for cDNA library construction and RNA-sequencing was done followed by de novo sequence assembly to provide a cost-effective and comprehensive means of transcriptome level information for M. oleifera. A total of 182,588 transcripts were generated in this study. Out of these transcripts 832 unigenes or .45% are involved in oil biosynthesis. The most numerous unigenes are those involved in fatty acid biosynthesis with 223 unigenes, fatty acid metabolism with 191 unigenes and triacylglycerol metabolism with 151 unigenes. There are 27 unigenes encoding for transcription factors involved in the regulation of oil biosynthesis gene expression. This is the first transcriptome resource ever reported for M. oleifera mature seed embryo. These unigenes are unmatched in protein databases for M. oleifera. Hence, the transcriptome resource for the M. oleifera Lam. mature seed embryo generated in this study will be useful for the mapping of oil biosynthesis related genes and the understanding of pathways which could possibly be used to improve seed yield and oil content of M. oleifera.


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1 - Ateneo de Manila University, Department of Biology, Loyola Heights,, Katipunan Avenue, Quezon City, 1108, Philippines

Keywords:
Transcriptome
Moringa
embryo
RNA-Seq.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:45 PM
Number: PGP017
Abstract ID:1361
Candidate for Awards:Genetics Section Poster Award


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