| Abstract Detail
Biochemistry, metabolism, carbon flux Karunakaran, Chithra [1], Lahlali, Rachid [1], Souter, Jodi R. [2], Dynes, James [1], Zuin, Lucia [1], Liu, Xia [1], Rosendahl, Scott [1], Regier, Tom [1], Todd, Christopher [2]. Mid infrared and soft X-ray spectroscopy techniques to understand nitrogen fixation in legumes. A good understanding of nitrogen fixation and associated processes is essential to develop legume crops suited for different growing regions or conditions. Currently, metabolic products of nitrogen fixation in legumes and other nutrients involved in the process are primarily analyzed by biochemical methods. Extraction processes vary and may be tedious or time-consuming, alter some metabolic compounds, and may not be suitable across species. Here, we present the advantages of combining mid-infrared and soft X-ray spectroscopy techniques as rapid methods to study nitrogen and other elements (sulfur, phosphorus, boron) in different parts of plant such as nodules, roots, and leaves. Soybean plants were grown under controlled conditions and fertilized with a defined nutrient solution at moderate or elevated manganese concentration. Plant tissues were harvested, freeze-dried, and ground into a fine powder that was used to collect the spectral data. The spectroscopy techniques revealed that the nitrogen content, and that of other elements, varied in soybean nodules, roots and leaves with managanese concentration in the nutrient solution and that manganese concentration altered the nitrogen forms in different parts of the soybean plant. Log in to add this item to your schedule
1 - Canadian Light Source, 44 Innovation Boulevard, Saskatoon, SK, S7N 2V3, Canada 2 - University of Saskatchewan, Department of Biology, 112 Science Place, W.P.Thompson Bld, Saskatoon, SK, S7N 5E2, Canada
Keywords: Soybean Nitrogen fixation mid infrared spectroscopy soft X-ray spectroscopy nitrogen sulfur phosphorous boron manganese.
Presentation Type: Poster:Posters for Topics Session: P Location: Hall D/The Shaw Conference Centre Date: Monday, July 27th, 2015 Time: 5:30 PM Number: PBC001 Abstract ID:1365 Candidate for Awards:None |