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Abstract Detail

Biochemistry, metabolism, carbon flux

Karunakaran, Chithra [1], Lahlali, Rachid [1], Souter, Jodi R. [2], Dynes, James [1], Zuin, Lucia [1], Liu, Xia [1], Rosendahl, Scott [1], Regier, Tom [1], Todd, Christopher [2].

Mid infrared and soft X-ray spectroscopy techniques to understand nitrogen fixation in legumes.

A good understanding of nitrogen fixation and associated processes is essential to develop legume crops suited for different growing regions or conditions. Currently, metabolic products of nitrogen fixation in legumes and other nutrients involved in the process are primarily analyzed by biochemical methods. Extraction processes vary and may be tedious or time-consuming, alter some metabolic compounds, and may not be suitable across species. Here, we present the advantages of combining mid-infrared and soft X-ray spectroscopy techniques as rapid methods to study nitrogen and other elements (sulfur, phosphorus, boron) in different parts of plant such as nodules, roots, and leaves.
Soybean plants were grown under controlled conditions and fertilized with a defined nutrient solution at moderate or elevated manganese concentration. Plant tissues were harvested, freeze-dried, and ground into a fine powder that was used to collect the spectral data. The spectroscopy techniques revealed that the nitrogen content, and that of other elements, varied in soybean nodules, roots and leaves with managanese concentration in the nutrient solution and that manganese concentration altered the nitrogen forms in different parts of the soybean plant.

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1 - Canadian Light Source, 44 Innovation Boulevard, Saskatoon, SK, S7N 2V3, Canada
2 - University of Saskatchewan, Department of Biology, 112 Science Place, W.P.Thompson Bld, Saskatoon, SK, S7N 5E2, Canada

Nitrogen fixation
mid infrared spectroscopy
soft X-ray spectroscopy

Presentation Type: Poster:Posters for Topics
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PBC001
Abstract ID:1365
Candidate for Awards:None

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