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Abstract Detail


Mort, Mark [1], Crawford, Daniel J. [1], Kelly, John K. [2], Santos-Guerra, Arnoldo [3], Menezes de Sequeira, Miguel [4], Moura, Mónica [5], Caujapé-Castells, Juli [6].

Multiplexed-shotgun-genotyping data resolve phylogeny within a very recently-derived insular lineage.

Insular endemic plants have long served as models for studying patterns and processes of evolution. However, phylogenetic studies of island plants frequently illustrate a decoupling of molecular divergence and ecological/morphological diversity, resulting in phylogenies lacking the resolution required to interpret patterns of evolution. Tolpis (Asteraceae) is widespread in Macaronesia, occurring in the Canary Islands (CI), Madeira, Azores, and Cape Verde (CV). Prior molecular phylogenetic studies have resolved several clades reflecting geography, including a CI-CV clade, comprising taxa from the five western-most CIs and CV. Members of CI-CV are distributed on substrates of broad geologic age, and vary in morphology, reproductive biology, and ecology. A new species in CI-CV was recently named, and studies suggest additional cryptic diversity. Despite extensive efforts, prior estimates of phylogeny for Tolpis have lacked the resolution and sampling necessary to provide a robust framework for evolutionary studies. The present study illustrates the utility of Multiplexed-Shotgun-Genotyping (MSG) for resolving relationships at various depths in this small, yet diverse insular plant lineage that had not been resolved with other molecular markers. Genomic libraries for 27 accessions of Macaronesian Tolpis were generated for genotyping individuals using MSG method, a form of RADseq. The resulting data files were processed using the program pyRAD, which clusters RAD loci within and between samples. Phylogenetic analyses of the aligned data matrix were conducted using RAxML. Analyses of MSG data achieved a highly resolved phylogeny with generally strong support, including the first robust inference of relationships within the highly diverse Canary Island clade of Tolpis. The present study illustrates the utility of MSG for resolving relationships in lineages that have undergone recent, rapid diversification resulting in extensive ecological and morphological diversity. We suggest that a similar approach may prove generally useful for other rapid, recent plant radiations where resolving phylogeny has been difficult.

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1 - University of Kansas, Ecology and Evolutionary Biology and Biodiversity Institute, 1200 Sunnyside Ave, Lawrence, KS, 66045, USA
2 - University of Kansas, Ecology and Evolutionary Biology, 1200 Sunnyside Ave, Lawrence, KS, 66045, usa
3 - Calle Guaidil 16, Urbanización Tamarco, Tegueste, Tenerife, Canary Islands, 38280, Spain
4 - Universidade da Madeira, Centro de Ciências da Vida, Campus da Penteada, Funchal, Portugal
5 - Universidade dos Açores, Departamento de Biologia, Rua da Mãe de Deus, Ponta Delgada, Açores, Portugal
6 - Jardin Botanico Canario "Viera y Clavijo"-Unidad Asociada CSIC, Cabildo de Gran Canaria, Camino al Palmeral 15, Tafira Alta, 35017, Las Palmas de Gran Canaria, Spain

genome sequencing
Canary Islands.

Presentation Type: Oral Paper:Papers for Topics
Session: 32
Location: Salon 11/The Shaw Conference Centre
Date: Tuesday, July 28th, 2015
Time: 8:15 AM
Number: 32002
Abstract ID:142
Candidate for Awards:None

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