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Abstract Detail

Host/Plant Pathogen Interactions and Plant Health Management

Brar, Gurcharn [1].

Fusarium head blight resistance QTLs Fhb1, Fhb2, and Fhb3 reduce disease severity by up to 50% in near-isogenic wheat lines developed by marker-assisted selection.

Fusarium head blight (FHB) is caused by various Fusarium spp., particularly F. graminearum, F. avenaceum and F. poae, and is the most serious disease affecting wheat (Tritichum aestivum L. and T. turgidum L.) across Canada. Quantitative trait loci (QTLs) associated with FHB resistance can be validated by quantifying their effects when in a common background such as in near-isogenic lines (NILs). Two single seed descent NIL populations were developed from the crosses CDC Go (susceptible)*4/04GC0139 (resistant) and CDC Alsask (susceptible)*4/04GC0137 (resistant). The near-isogenic line populations were developed by backcrossing F1 plants to CDC Go or CDC Alsask. At the BC4F1 generation, each NIL was screened by microsatellite (SSR) markers associated with Fhb1, Fhb2 and Fhb3. Approximately 1,900 BC2F1 and 1,200 BC3F1 hybrid seeds were genotyped. The three major QTLs were successfully backcrossed to CDC Go and CDC Alsask with the recovery of all eight possible combinations. The FHB resistant QTL derived from Sumai 3, and then introgressed into CDC Go and CDC Alsask, reduced the severity of infection in greenhouse trials by up to 50% and 25%, respectively. In an inoculated field nursery, the Fusarium head blight visual rating index in lines carrying all three QTLs (Fhb1+Fhb2+Fhb3) was 50% that of the recurrent parent (CDC Go). The results suggest that marker-assisted selection (MAS) to stack Fhb1, Fhb2, and Fhb3 QTLs is effective in improving FHB resistance relative to the susceptible recurrent wheat parent.

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1 - University of Saskatchewan, Plant Sciences, 51 Campus Dr., Agriculture Building, Saskatoon, SK, S7N 5A8, Canada


Presentation Type: Oral Paper:Papers for Topics
Session: 46
Location: Salon 2/The Shaw Conference Centre
Date: Tuesday, July 28th, 2015
Time: 1:45 PM
Number: 46002
Abstract ID:157
Candidate for Awards:CPS Best Student Presentation Awards

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