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Abstract Detail

Host/Plant Pathogen Interactions and Plant Health Management

Singh, Khushwant [1], Wenzlova, Jana [1], Mazakova, Jana [1], Winter, Mark [2], Koopmann, Birger [2], von Tiedemann, Andreas [2], Zouhar, Miloslav [1], Rysanek, Pavel [1].

Cyclophilin A: A Key determinant in distinguishing Oilseed rape fungal pathogen Leptosphaeria maculans and L. biglobosa Causing ‘Blackleg’.

‘Blackleg’ is the most economical important disease on canola (Brassica napus). The disease is caused by the fungal species complex Leptosphaeria maculans and L. biglobosa. L. maculans causes more severe symptoms in comparison to L. biglobosa. However, several studies have shown that L. maculans is much more aggressive on oilseed rape (Shoemaker and Brun, 2001). This phenomenon can only in small parts be explained by its attribute to produce the host-unspecific toxins sirodesmins (Sock and Hoppe, 1999), which are not produced by L. biglobosa. However, it is still unclear which main factors underlie these huge differences of aggressiveness. Cyclophilins are highly conserved family responsible to determine the level of virulence (Viaud et al., 2002). Here we performed functional characterization of cyclophilin A (CypA) from L. maculans and L. biglobosa in order to delineate its precise role in virulence. In-silico analyses of sequences followed by cloning of cyclophilin A (Cyp4) in various isolates showed the compelling differences between two species at sequence level as well. In addition, expression levels of the Cyp4 in the mycelium found to be relatively high in L. maculans as compared to L. biglobosa. However, the expression analyses not only demonstrated a significant difference among species but also at intraspecific variation. Furthermore, pigmentation and sirodesmin analyses distinguished L. maculans and L. biglobosa isolates. Host-pathogen interaction on various oilseed cultivars was also carried and thus demonstrated that different isolates exhibited different level of pathogenicity. In addition ongoing ad planta studies may further support our hypothesis that cyclophilins and their expression may explain the difference in virulence on oilseed rape against L. maculans and L. biglobosa (Unpublished data). References: Shoemaker, R.A, and H. Brun, 2001. The teleomorph of the weakly aggressive segregate of Leptosphaeria maculans. Can J Bot 79: 412–419. Viaud, M.C, P.V. Balhadere, N.J. Talbot, 2002. A Magnaporthe grisea cyclophilin acts as a virulence determinant during plant infection. Plant Cell 14: 917–930.

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1 - Czech University of Life Sciences Prague, Department of Plant Protection, Prague, 16521, Czech Republic
2 - Georg-August University Göttingen, Department of Crop Sciences, Göttingen, 37077, Germany


Presentation Type: Poster:Posters for Topics
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PPA007
Abstract ID:160
Candidate for Awards:CPS Best Student Presentation Awards

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