Abstract Detail

Recent Topics Posters

Licayan, Richard [1], Healy, Heather [2], Giurleo, Daniel [3], Del Rosario, Romeo [4], Del Rosario, Romeo [4], Palmes, Nenita [4], Juliani, Rodolfo [5], Wu, Qingli [6], Simon, James [1].

Nutrient Analysis, Antioxidant Capacity, and Cytotoxicity of Eleven Selected Herbal Weeds of Philippine Origin.

The world's supply of food and micronutrients is out of balance. According to the World Health Organization, approximately 162 million of the world’s children are stunted, and 30 percent of the world’s population suffers from one or more micronutrient deficiencies. This study sought to ascertain the elemental components, leaf polyphenols, total carotenoids, total protein, antioxidant capacity, and cytotoxicity of 11 selected herbal weeds of Philippine origin including Coccinia grandis, Mikania micrantha, Ruellia tuberose, Merremia peltata, Chromolaena odorata, Clitorea ternatea, Passiflora foetida, Mumordica chuchinensis, Ipomoea pes caprae, Ipomoea cairica, and Ipomoea lacunos. Quantitative screening of the leaf polyphenol content of the vine weeds in terms of mg of gallic acid equivalent was tested using Folin-ciucalteu assay. Absorbance was read via Synergy HT multi-well spectrophotometer at 752 nm. The total carotenoids in the herbal weeds leaf extract were quantified using a spectroscopic method at 450 nm. The antioxidant activity of the samples was determined by ABTS assay expressed in terms of mg of Trolox equivalent. The cytotoxic activity of the plant leaf extracts was tested using brine shrimp lethality assay. The 11 herbal weeds have high calcium, potassium, phosphorus, and magnesium content.  Chomolaena odorata had the highest polyphenolic content with an equivalent value of 119.26 mg gallic acid equivalent (GAE) per gram of dry weight while Coccinia grandis had a high total carotenoid content (9, 160.23 IU per gram).  Clitorea ternatea had the highest total protein content (22.28 mg albumin/ gram dry weight). The highest antioxidant activity was observed in C. grandis with an equivalent of 6.927 mg Trolox equivalent antioxidant capacity (TEAC) per gram of dried material. Results from the cytotoxicity screens showed that C. odorata leaf extract was the most lethal against the brine shrimp with LC50 values of 1.0 μg/mL. These herbal weeds can be rich sources of nutraceutical components and maybe used for functional food and pharmaceutical products for prevention and treatment of various types of chronic and degenerative diseases caused by oxidative stress.

1 - Rutgers University, New Use Agriculture & Natural Plant Products Program, Department of Plant Biology and Pathology, 59 Dudley Road, Foran Hall, Rutgers University, New brunswick, NJ, 08901, United States
2 - Rutgers University, Food Science, 59 dudley road, ew brunswick, NJ, 08901, United States
3 - Rutgers University, New Use Agriculture & Natural Plant Products Program, Department of Plant Biology and Pathology, 59 Dudley Road, Foran Hall, Rutgers University, New brunswick, NJ, 08901, USA
4 - Mindanao University of Science and Technology, Chemistry, C.M. Recto Avenue, Lapasan Highway, Cagayan de Oro, 9000, Philippines
5 - 315 Wayne Street, Highland Park, NJ, 08904-2717, USA
6 - Rutgers University, New Use Agriculture & Natural Plant Products Program, Department of Plant Biology and Pathology, 59 Dudley road, New brunswick, NJ, 08901, United States

Keywords:
Antioxidant capacity
carotenoids
cytotoxicity
elemental analsysis
polyphenols
protein
Philippine herbal weeds.

Presentation Type: Recent Topics Poster
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PRT001
Abstract ID:1777
Candidate for Awards:None