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Abstract Detail

Genomics / Proteomics

Uhrig, R. Glen [1], Echevarría-Zomeño, Sira [1], Grossmann, Jonas [2], Roschitzki, Bernd [2], Gruissem, Wilhelm [1].

Examination of clock related post-translational protein regulation through quantitative proteomics.

Classically, the circadian clock has been described as a series of modular molecular encoded negative feedback loops that precisely regulate global gene expression in a coordinated 24 hour cycle. As a result, the circadian clock is responsible for controlling numerous physiological processes central to plant growth and survival. However, the clock relies on various signal inputs for entrainment and accurate time-keeping. To date, the vast majority of circadian clock studies have relied on the changing transcriptome to infer molecular connections between the circadian clock and observable plant phenotypes. However, recent findings have shown a disconnect between changing transcripts and proteins in circadian clock mutants, indicating that clock regulatory mechanisms beyond gene expression are responsible for communicating retrograde and anterograde circadian clock signals. At the same time, reversible protein post-translational modifications (PTMs) play an essential role in regulating protein activity, subcellular localization and protein-protein interactions in the cell. Protein phosphorylation represents the most prolific of the known PTMs, having been characterized to regulate upwards of 70% of proteins in Eukaryotes. In plants, protein phosphorylation has also been shown to regulate core-clock proteins. Using a variety of quantitative proteomics approaches we endeavored to uncover novel components of the circadian clock though assessment of total protein and protein PTM changes over a 24 hour time-course of Arabidopsis thaliana rosettes.

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Related Links:
ETH Functional Genomics Center
Gruissem Lab

1 - ETH Zurich, Department of Biology, Universitatstrasse 2, LFW Building, Zurich, 8092, Switzerland
2 - ETH Zurich Functional Genomics Center , Winterthurerstr. 190, Zurich, 8057, Switzerland

Mass Spectrometry
Circadian Clock.

Presentation Type: Oral Paper:Papers for Topics
Session: 48
Location: Salon 19/20/The Shaw Conference Centre
Date: Tuesday, July 28th, 2015
Time: 2:45 PM
Number: 48006
Abstract ID:178
Candidate for Awards:Margaret Menzel Award

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