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Abstract Detail

Recent Topics Posters

Mei, Angela [1].

Modulation of autonomous selfing success and the timing of self-pollination under water stress in Collinsia heterophylla.

Collinsia heterophylla (Family: Plantaginaceae), a mixed-mated, annual wildflower, native to southern California, maintains outcrossing by two sexual interference mechanisms – physical separation of anther-stigma placement (herkogamy) and temporal separation of anther-stigma maturation (dichogamy), resulting in limited selfing success. Previous results indicate that seed production by autonomous selfing is increased under water stress. Flowers of water-stressed plants exhibit reduced herkogamy that could facilitate easy self-pollen deposition. In addition, anther dehiscence is slightly advanced, further augmenting chances of self-pollination under water stress. To understand the detailed mechanisms of such increased selfing and the timing of self-pollination, in the current study, we investigated the expression of dichogamy by determining the sequence of anther dehiscence in relation to stigmatic receptivity and changes therein. Plants were allocated to control and water stress. Control plants received water twice a week and water stressed plants once a week. Within each of these treatments tagged flowers were observed for the day of anther dehiscence. Experimental removal of undehisced anthers and resulting seed production allowed us to determine the timing of self-pollen deposition and anthers contributing to selfing success. Stigma from flowers were collected each day an anther dehisced, and analyzed for germinated stigmatic pollen to determine differences in autonomous self-pollen deposition and stigmatic receptivity. Our results indicate autonomous self-pollen deposition on stigma was higher under water stress and occurred a day earlier than in control. Confirmatory studies on stigmatic receptivity and other mechanisms facilitating breeding system modulation under abiotic stress are underway. 

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1 - Colorado State University, Biology, 9928 W Mesa Vista Ave, , Las Vegas, NV, 89148, USA

drought stress
Water stress
Collinsia heterophylla

Presentation Type: Recent Topics Poster
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PRT005
Abstract ID:1781
Candidate for Awards:None

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