Abstract Detail

Recent Topics Posters

Sandha , Gursahib Singh [1], Sirari, Asmita [2], Sharma, Abhishek [2], Vandenberg, Albert [3].

Role of storage conditions in seed transmission of Yellow Mosaic Disease (YMD) in mung bean (Vigna radiata L.).

  Mung bean (Vigna radiata L.) is an important food legume in the tropical and subtropical parts of world.  Among its numerous abiotic stresses, Yellow Mosaic Disease (YMD) is one of the most devastating diseases. YMD affects the pods of susceptible genotypes resulting in small sized seeds and lower yield.  The objective was to determine if YMD in mung bean was transmitted through seeds.   Mature pods of mung bean variety RMG 353 (susceptible check) were harvested and stored at ambient temperature till next cropping season.  The seeds were sown under whitefly proof net cages and in open in next season.  The caged plants and field grown plants were regularly monitored for symptoms.  YMD was observed on plants sown in the open after 45-50 days from sowing.  Plants sown under controlled condition did not develop symptoms.  The green pods and mature pods showing YMD symptoms were collected to detect the virus through rolling circular amplification (RCA) and polymerase chain reaction (PCR).  The virus was detected in green pods with YMD symptoms.  The seeds from the mature diseased pods were kept under two storage conditions, one at room temperature and the other at 4ºC.  The seeds kept at room temperature and 4ºC were monitored for the presence of TMD virus at monthly intervals.  The virus was detected in seeds for up to one month for storage at room temperature for up to two months of storage at 4ºC. The results suggested that seed does not play a role in YMD transmission but storage conditions and duration can.  

1 - University of Saskatchewan, 214 Cumberland Avenue North, Saskatoon, Saskatchewan, S7N 1M4, Canada
2 - Punjab Agricultural University, Plant Breeding & Genetics, Ludhiana, Punjab, India
3 - University of Saskatchewan, 51 Campus Dr., Saskatoon, Saskatchewan, S7N 5A8, Canada

Keywords:
YMD.

Presentation Type: Recent Topics Poster
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PRT013
Abstract ID:1796
Candidate for Awards:None