| Abstract Detail
Recent Topics Posters Peng, Fred [1], Hu, Zhiqiu [2], Yang, Rong-Cai [2]. Comparative Genomic Analysis of Flowering-related Genes in Arabidopsis, Wheat and Barley. The timing of flowering is one of the most critical agronomic traits influencing grain yield and quality in the two important temperate cereal crops wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.). The purpose of this presentation is to investigate the usefulness of a bioinformatics approach to identification of flowering-related genes in these crop species. A list of 190 Arabidopsis (Arabidopsis thaliana (L.) Heynh.) genes involved in flowering pathways such as photoperiod, circadian clock, and vernalization was initially produced through literature searches. We then identified 900 and 275 putative orthologs of Arabidopsis flowering genes in wheat and barley, respectively, using a two-stage approach that combines reciprocal BLAST searches and OrthoMCL clustering. A total of 144 orthologous groups were clustered with OrthoMCL for the flowering-related genes in these three species. Domain and phylogenetic analyses further validated our approach to orthology prediction. Analysis of publicly available microarray data sets for in silico expression profiling of flowering-related genes showed that many orthologous genes in wheat and barley exhibited similar expression patterns among 13 tissues or developmental stages, yet with additional wheat paralogs in the same ortholog group being unexpressed or expressed differently. The putative orthologous sequences we annotated by homology-based inference using Arabidopsis as reference can be further confirmed and incorporated into molecular breeding for early flowering and maturing wheat and barley in western Canada and other high-latitude regions.
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1 - Alberta Agriculture and Forestry, Feed Crops Branch, 7000 - 113 Street, Edmonton, AB, T6H 5T6, Canada
2 - University of Alberta, Department of Agricultural, Food and Nutritional Science, 410 Agriculture/Forestry Centre, Edmonton, AB, T6G 2P5, Canada
Keywords:
flowering time
ortholog prediction
Arabidopsis thaliana
Triticum aestivum
Hordeum vulgare.
Presentation Type: Recent Topics Poster
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PRT015
Abstract ID:1798
Candidate for Awards:None |
