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Abstract Detail



Recent Topics Posters

Sedaghatpour, Maryam [1], Oldham, Karoline [2], Weeks, Andrea [3].

Microsatellite markers for estimating the population genetic diversity of the hemiparasitic wildflower, Melampyrum lineare (Orobanchaceae).

Melampyrum lineare Desr. (narrowleaf cowwheat) is a hemiparasitic, annual flowering plant native to North America. It obtains water and nutrients by penetrating the roots of its adjacent vascular host species, such as maple trees, pine trees, and members of Ericaceae. Due to this physiological dependency, M. lineare is limited to the geographical range of its hosts. Within the United States, its range extends from the southern Appalachian mountains to throughout the northeastern states and westward to Minnesota, with disjunct populations in Idaho and Montana.  Within Canada, it is widely distributed at lower latitudes from Newfoundland to Vancouver Island as well as further north along the more temperate Pacific coast. In the face of a changing climate, understanding the genetic diversity of M. lineare is important for its conservation, particularly within the southern-most extent of its distribution and in US states where it is listed as rare and threatened, and for illuminating how this interdependent species tracked its host species over geological time. Thirty-two microsatellite primer pairs originally designed for Melampyrum sylvaticum, a European species, were tested on a single individual of M. lineare using a modified PCR protocol. Primer pairs, MsO66P, MsO70M, MsG2, and MsB58, amplified successfully and produced DNA fragments ranging from ca. 125 to ca. 500 base pairs. The four markers were then tested on 12 geographically distinct individuals of M. lineare from Georgia to Maine to determine allelic variation of the microsatellite regions among populations. Initial results from agarose gel electrophoresis indicated some allele size variation and heterozygosity of loci, however the DNA fragments must be sequenced to determine their precise length and base pair composition. Once this information is verified, we will then move to the final data collection stage for over 400 individuals from 50 populations of Melampyrum lineare on the East Coast of the United States as well as individuals that will be collected from Alberta and British Columbia provinces in August 2015. 


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1 - George Mason University , Department of Environmental Science and Policy, Fairfax, VA, 22030, USA
2 - George Mason University , School of Systems Biology, 4400 University Drive, MS 3E1, Fairfax, VA, 22030, USA
3 - George Mason University, Biology, 4400 University Dr., MSN 3E1, Fairfax, VA, 22030-4444, USA

Keywords:
genetic diversity
population genetics
Genetic marker
genetic variation
Orobanchaceae
microsattelite
Melampyrum
hemiparasitic.

Presentation Type: Recent Topics Poster
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PRT036
Abstract ID:1821
Candidate for Awards:None


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