Abstract Detail

Recent Topics Posters

Ljubotina, Megan [1], Bao, Tan [2], Melenka, Garrett W. [3], Carey, Jason P. [4], Cahill Jr., James F. [5].

To the root of the problem: a novel approach to root imaging using digital image correlation.

Studies on belowground plant morphology, root placement, and habitat occupancy are often limited by the difficulty of imaging the root system. We describe a new root imaging technique using digital image correlation (DIC) to track the movement of reflective particles in the substrate. By using successive photographs to compare changes in the relative displacement of these particles, we are able to infer growth of roots over time, including those that are otherwise visually obstructed by substrate. Here, Abutilon theophrasti, Solanum lycopersicum, and Helianthus annuus specimens were grown in clear acrylic pots. Plants were photographed daily over the course of 3 weeks and the resulting photo series were analysed using DIC to calculate strain, a measure expressing displacement relative to a reference length. Excavation of the roots confirmed a close correlation between measures of strain and the placement of roots, including those roots that were not initially visible through the transparent wall of the pot. DIC analysis is a non-invasive method that allows for improved flexibility in the biological questions asked when compared with methods using artificial substrates such as gels, since aspects of natural soil environments (e.g. microbial communities, nutrient patches, and variability in soil texture) are easily produced. The technique possesses potential for addressing questions about belowground ecological interactions such as competition and nutrient acquisition that are also key in agronomy, as well as providing information about the root system’s structure.

1 - University of Alberta, Department of Biological Sciences, CW 405, Biological Sciences Building, Edmonton, AB, T6G 2E9, Canada
2 - University of Alberta, Department of Biological Sciences, CW 405, Biological Sciences Bldg., Edmonton, AB, T6G 2E9 , Canada
3 - University of Alberta, Department of Mechanical Engineering, 4-9 Mechanical Engineering Building, Edmonton, AB, T6G 2G8, Canada
4 - University of Alberta, 4-9 Mechanical Engineering Building, Edmonton, AB, T6G 2G8, Canada
5 - University Of Alberta, Department Of Biological Sciences, CW405 Bio Sci Bldg, Edmonton, AB, T6G 2E9, Canada

Keywords:
none specified

Presentation Type: Recent Topics Poster
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PRT042
Abstract ID:1828
Candidate for Awards:None