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Abstract Detail

Recent Topics Posters

Berger, Brent [1], Riciglicano, Vincent [2], Sessa, Emily Butler [3], Gardner, Andrew [4], Shepherd, Kelly [5], Han, Jiahong [6], Jabaily, Rachel [7], Howarth, Dianella [2].

Using genome skimming to uncover coding and regulatory sequences of floral symmetry genes in Core Goodeniaceae.

Genome skimming has emerged as a cost effective technique for retrieving genic regions known to occur in high abundance (chloroplast, mitochondria, ribosomal DNA and spacers). In plants, this technique has greatly aided our understanding of phylogenetic relationships because of the ability to assemble nearly complete plastomes across diverse groups of flowering plants. Within Core Goodeniaceae (Asterales), we previously used genome skimming to produce plastome sequences from 24 species to resolve backbone relationships. The Core Goodeniaceae contains roughly 330 species that are predominately native to Australia and the Pacific Islands. This group includes monotypic Brunonia australis, which is sister to two major clades: Goodenia s.l. (with 5 embedded genera) and Scaevola s.l. (with 1 embedded genus). Floral variation in Core Goodeniaceae likely plays a role in the extensive polyphyly in the taxonomy of the group. This variation is particularly apparent in shifts in floral symmetry and shape. We are now using these low coverage data to mine other genes of interest, particularly those known to play a role in floral symmetry, such as CYCLOIDEA-like (CYC-like) genes. The datasets were assembled de novo and CYC-like regions were identified with BLAST. We have found an average of 4 gene regions from each genome. CYC-like genes are roughly 900 bps with two conserved domains, TCP and R. Most available sequences have been generated using primers in the conserved domains, sequencing only a third of the gene, and therefore are missing up- and down-stream regions. Using 24 separate species, even without genomic sequencing depth, has allowed us to generate a scattered matrix (each region from only a few species) with coding and even regulatory regions. Specific primers can then be generated to fill in the matrix. This is a useful approach to study upstream regulatory regions and full length coding sequences from a clade of non-model plants, allowing for comparisons between morphological shifts and molecular evolution.

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1 - St. John's University, Department of Biological Sciences, 8000 Utopia Pkwy, Jamaica, NY, 11439, USA
2 - St. John's University, Biological Sciences, 8000 Utopia Pkwy, Jamaica, NY, 11439, USA
3 - University Of Florida, Department of Biology, Box 118525, Gainesville, FL, 32611, USA
4 - Rhodes College, Department of Biology, 2000 N. Parkway, Memphis, TN, 38112, USA
5 - Western Australian Herbarium, Department of Parks and Wildlife, Kensington, Western Australia, 6983, Australia
6 - St. John's University, Department of Biological Sciences, 8000 Utopia Parkway, Jamaica, NY, 11439, USA
7 - Rhodes College, 2000 N. Parkway, Memphis, TN, 38112, United States

none specified

Presentation Type: Recent Topics Poster
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PRT051
Abstract ID:1837
Candidate for Awards:None

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