| Abstract Detail
Recent Topics Posters Stone , James David [1], Sloan, Dan [2], Štorchová, Helena [3]. Comprehensive survey of mitochondrial transcription and RNA editing in gynodioecious Silene vulgaris. Using an RNA-seq approach, we provide a comprehensive view of transcription in floral buds harvested from female and hermaphrodite Silene vulgaris (Caryophyllaceae). Beyond coding sequences and UTRs, we found substantial transcription of group II intron sequences as well as occasional elevated transcription in ORFs and long noncoding RNAs. A comparison of RNA-seq data with the mt genome revealed expected and unexpected RNA editing events across the mt genome. We compare observed editing events with predictions based on conserved coding sequences as well as events observed in Arabidopsis. We detected numerous editing events far from annotated genes, most presumably due to off-target editing, but a few within noncoding “islands” of transcriptional activity. We also note conserved silent editing sites and patterns of edit site loss that may enrich our understanding of editing factor binding rules. Our results highlight the relaxed transcriptional control in plant mitochondrial and shed new light on the mitochondrial transcriptional processes in the differentiating tissues of a gynodioecious plant.
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1 - Institute of Botany, Czech Academy of Science, 1 Zamek, Průhonice, 252 43, Czech Republic
2 - Colorado State University, Biology, Fort Collins, CO, 80523, USA
3 - Institute of Experimental Botany, Czech Academy of Science, Rozvojová 313, Prague, 165 02, Czech Republic
Keywords:
gynodioecy
pentatricopeptide
ppr
RNA-Seq
editome
cms
cytoplasmic male sterility.
Presentation Type: Recent Topics Poster
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PRT056
Abstract ID:1842
Candidate for Awards:None |
