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Abstract Detail

Systematics Section/ASPT

Arbizu, Carlos [1], Simon, Philipp [1], Ruess, Holly [1], Spooner, David [1].

Phylogeny of Daucus broteri, D. guttatus, and related species.

In our previous study using 94 nuclear orthologs, the status of Daucus broteri and D. guttatus was unresolved, as the data supported three taxa. In the present study, a set of ten nuclear ortholog markers with 11,227 bp aligned length were used to infer the phylogeny of a greater subset of accessions to include 85 accessions of 11 species and three subspecies of Daucus, and one close outgroup. A near parallel set of accessions were used for morphological analyses of 42 germplasm accessions planted in a common garden in Madison, Wisconsin. Morphological analyses included character state distributions, stepwise discriminant analyses, canonical variants analyses, linear discriminant analysis, and hierarchical cluster analyses. Our maximum parsimony DNA tree is highly resolved, with 100% bootstrap support for most of the external and many of the internal clades. They grouped multiple accessions of many different species as monophyletic with strong support, but failed to support D. guttatus and D. broteri individually. Our result indicates there are four clades with very good bootstrap support supporting four different taxa. Concordant with molecular analyses, most species form well defined phenetic groups. To date two of these entities are associated with the types of D. conchitae and D. guttatus, and we are continuing to resolve the nomenclature. Our research highlights some difficult species group in Daucus, and discovered misidentifications in germplasm collections. It confirms a new useful subset of nuclear orthologs and morphological characters for taxonomic studies of Daucus.

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1 - USDA Agricultural Research Service, University of Wisconsin-Madison, 1575 Linden Dr, Madison, WI, 53706, USA

none specified

Presentation Type: Oral Paper:Papers for Sections
Session: 49
Location: Salon 4/The Shaw Conference Centre
Date: Tuesday, July 28th, 2015
Time: 2:30 PM
Number: 49005
Abstract ID:226
Candidate for Awards:George R. Cooley Award

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