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Abstract Detail



Genetics Section

Jin, Xiaojie [1], Chi, Dawn [1], Zhang, Bo [1], Xue, Allen [2], Cao, Wenguang [2], Fedak, George [2].

Wheat germplasm development by gene pyramiding for ressitance to Ug99.

Most of the current stem rust resistance genes (Sr) in Canadian wheat varieties are ineffective against the Puccinia graminis f.sp. tritici Ug99 races which pose a major threat to wheat production worldwide. Several stem rust resistance genes, including Sr33, Sr35, Sr36, SrCad /Sr42 and Sr43, are effective against the Ug99 race TTKSK. Although Sr36 became ineffective against the Ug99 race TTTSK, it is still potentially useful for pyramiding these genes to develop durable stem rust resistant germplasm. For this purpose, we made two crosses among RL5405 (Sr33), RL6099 (Sr35), Lang (Sr36), AC Cadillac (SrCad/Sr42) and RWG34 (Sr43) which contain the corresponding Sr genes. A total of 54 doubled haploid (DH) lines were produced from F1 hybrids involving (AC Cadillac/Lang)//(RWG34/RL5405) and 82 DH lines obtained from RWG34/RL5405//RL6099. Twelve genotypes with different Sr gene combinations including Sr33+Sr36+SrCad/Sr42+Sr43, Sr33+Sr36+SrCad/Sr42, Sr33+Sr36+Sr43, Sr33+SrCad/Sr42+Sr43, Sr36+SrCad/Sr42+Sr43, Sr35+Sr33+Sr43, Sr33+Sr36, Sr33+Sr43, Sr36+SrCad/Sr42, Sr36+Sr43, Sr35+Sr33, Sr35+Sr43 were verified by linked PCR markers. Another population with 63 DH lines was derived from the combination (Hoffman*2/RL6099)//(Hoffman*2/Lang) which was made to improve Hoffman’s resistance to Ug99. Seventeen of 63 DH lines contained both Sr35 and Sr36 genes, indicated that the combination of Sr35+Sr36 was pyramided in the Canadian wheat cultivar ‘Hoffman’ and these lines may be used for development of resistant cultivars to Ug99 in Canada.


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1 - Agriculture and Agri-Food Canada, Eastern Cereal and Oilseed Research Centre, Ottawa, ON, K1A 0C6, Canada
2 - Agriculture and Agri-Food Canada, Eastern Cereal and Oilseed Research Centre, 960 Carling Avenue, Ottawa, ON, K1A 0C6, Canada
3 - Agriculture and Agri-Food Canada, Eastern Cereal and Oilseed Research Centre, 960 Carling Avenue, Ottawa, ON, K1A 0C6, Canada
4 - Agriculture and Agri-Food Canada, Eastern Cereal and Oilseed Research Centre, 960 Carling Avenue, Ottawa, ON, K1A 0C6, Canada

Keywords:
Stem rust
Ug99
wheat
gene pyramids.

Presentation Type: Poster:Posters for Sections
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PGN004
Abstract ID:236
Candidate for Awards:None


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