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Abstract Detail



Genetics Section

Fedak, George [1], Wolfe, Danielle [1], Chi, Dawn [2], Cao, Wenguang [1], Xue, Allen [3].

Process of transferring FHB resistance from Thinopyrum elongatum to spring wheat.

Germplasm with good FHB resistance will normally contain a number of major resistance QTL plus a number of minor QTL-all spread over the entire genome. It was thus unusual to find an aneuploid containing a single chromosome (7E) introgressed from Thinopyrum elongatum (2x) to have a high level of FHB resistance (9.6% infected florets following inoculation, compared to the parental cultivar at 57.5%). The Ph1b mutant is being used to induce chromosome pairing and recombination between 7E and a wheat chromosome, and thus transfer the resistance. The Ph1b mutant was crossed and backcrossed once to the three substitution lines 7E (7A), 7E (7B) and 7E (7D), giving 556 BC1 seeds. These seeds were screened with marker PSR574, specific for Ph1b, and 43% of the backcross progeny were found to be homozygous recessive for Ph1b.The plants that were homozygous recessive for Ph1b were inoculated with Fusarium graminearum and meiosis studied in them. Progeny from resistant plants that showed complete chromosome pairing at meiosis are being evaluated with chromosome 7E-specific markers to identify plants with the smallest 7E introgressions. Following mapping, the resistance QTL will be used to enhance FHB resistance in wheat breeding programs.


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1 - Agriculture and Agri-Food Canada, Eastern Cereal and Oilseed Research Centre, Ottawa, ON, K1A 0C6, Canada
2 - Agriculture and Agri-Food Canada, Eastern Cereal and Oilseed Research Centre, 960 Carling Ave., Ottawa, ON, K1A 0C6, Canada
3 - Agriculture and Agri-Food Canada, Eastern Cereal and Oilseed Research Centre, 960 Carling Avenue, Ottawa, ON, K1A 0C6, Canada

Keywords:
wheat
meiotic pairing control genes
Thinopyrum elongatum
fusarium head blight.

Presentation Type: Poster:Posters for Sections
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PGN005
Abstract ID:237
Candidate for Awards:None


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