Abstract Detail

Agronomy

Cober, Elroy [1], Molnar, Stephen [1], Charette, Martin [1].

Genetic Control of High Seed Protein in Short Season Soybean.

High seed protein is typically difficult to introgress into soybean without an accompanying reduction in seed yield. In previous studies, AC Proteus was used as high protein source and was found to produce populations without high protein low yield associations. Five high x low protein RIL populations were evaluated for use in either quantitative trait loci (QTL) or bulk segregant analyses (BSA) following phenotyping in the field. QTL analysis found two major QTL and these were independent of seed yield QTL. QTL-HP-20 at Satt496 on chromosome 20 was detected by BSA at Satt496 in three of the four populations, and by BSA at the adjacent marker Satt587 in the fourth population. Additional positive BSA results at flanking markers support the hypothesis that this locus is likely the major locus for Protein Content in all five populations, which have shared or related parentage. The related shoulder peak at Satt419 and Satt562 on chromosome 20 was also detected by BSA in three of the four populations. AC Proteus carries the Satt496 “B” allele associated with high protein but does not carry the high protein “B” allele at Satt419. QTL-HP-15 was found at Satt213 on chromosome 15. BSA at the flanking marker Satt411 was positive for two of the four populations, while the high protein parent’s allele was fixed in the other two populations. Again this is consistent with the hypothesis that this locus is significant for achieving high seed protein in all five populations. BSA found one unique locus not reported in the 35 previous seed protein studies catalogued on SoyBase.

1 - Agriculture and Agri-Food Canada, Eastern Cereal and Oilseed Research Centre, 960 Carling Ave, Ottawa, ON, K1A 0C6, Canada

Keywords:
Soybean
Glycine max
seed protein.

Presentation Type: Oral Paper:Papers for Topics
Session: 37
Location: Salon 2/The Shaw Conference Centre
Date: Tuesday, July 28th, 2015
Time: 10:45 AM
Number: 37010
Abstract ID:238
Candidate for Awards:None