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Abstract Detail



Systematics Section/ASPT

Gostel, Morgan [1], Weeks, Andrea [2].

Species-level phylogenetic resolution of Madagascan Commiphora (Burseraceae) revealed by multilocus target enrichment.

Existing molecular markers for phylogenetic reconstruction in the myrrh genus, Commiphora Jacq., have proven insufficient for complete species-level resolution of the endemic Madagascan clades. These lineages, which represent four separate introductions and comprise 44 species, are distributed in the dry deciduous forest and spiny bush along the latitudinal extent of the island. In order to improve phylogenetic resolution in these clades and test biogeographic hypotheses of species evolution in Madagascar, we have developed and screened a set of primers for 192 loci. Loci were identified using a transcriptome of Bursera simaruba aligned to a set of putative target loci from the Arabidopsis thaliana genome. We screened validated loci across 38 species using microfluidic PCR, which amplified the loci simultaneously, followed by parallel sequencing on the Illumina platform. We report our protocol, phylogenetic analyses of the individual loci and progress in using these novel sequence data to reconstruct the evolutionary history of this ecologically dominant group of trees in Madagascar.


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1 - George Mason University, Environmental Science And Policy, David King Hall, MSN 5F2, 4400 University Dr., Fairfax, VA, 22030-4444, USA
2 - George Mason University, Biology, 4400 University Dr., MSN 3E1, Fairfax, VA, 22030-4444, USA

Keywords:
High-throughput Sequencing
target enrichment
MiSeq
multilocus phylogenetics.

Presentation Type: Oral Paper:Papers for Sections
Session: 19
Location: Salon 9/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 2:00 PM
Number: 19003
Abstract ID:270
Candidate for Awards:None


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