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Abstract Detail



Phylogenomics

Beck, James [1], Semple, John [2].

Next generation sampling: obtaining genomic data from large sets of herbarium specimens.

Although a time is quickly approaching when powerful genomic datasets can be obtained and analyzed in any group of organisms, systematists remain fundamentally constrained by access to samples. While rewarding, fieldwork is expensive and time consuming, and collecting material for all but the smallest groups is well beyond the scope of many projects. We propose a solution- "Next Generation Sampling" involves extracting DNA and obtaining genetic/genomic datasets from large sets of natural history collections under the guidance of expert taxonomists. This approach allows robust sampling to be achieved rapidly and ensures the critical link between taxonomy and DNA. As a test case, we report preliminary results from the goldenrods (Solidago L.), a large plant genus that has resisted efforts at species delimitation and phylogeny reconstruction due to its size (ca. 120 species) and low levels of sequence divergence. Preliminary results clearly establish that genomic datasets can be obtained from goldenrod museum specimens of a wide age range, and that these data provide strong signal for species delimitation and phylogeny reconstruction in this notoriously difficult genus.


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1 - Wichita State University, Biological Sciences, 1845 Fairmount, 537 Hubbard Hall, Wichita, KS, 67260, USA
2 - University Of Waterloo, Department Of Biology, 200 University Ave., Waterloo, ON, N2L 3G1, Canada

Keywords:
Solidago
Species delimitation
phylogenomics
genotyping by sequencing
herbarium specimen.

Presentation Type: Oral Paper:Papers for Topics
Session: 32
Location: Salon 11/The Shaw Conference Centre
Date: Tuesday, July 28th, 2015
Time: 9:15 AM
Number: 32006
Abstract ID:274
Candidate for Awards:None


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