Abstract Detail

Mycological Section

Lopez-Carrasco, Rosa Angelina [1], Gonzalez-Davis, Oscar [2], Clark-Ordoñez, Isadora [3].

Candida yeast distribution in oral cavity isolates and their sensitivity to Fluconazole and Amphotericin B.

Candida species are ubiquitous organisms in nature and some are part of the natural human biota, where they grow in skin and mucous membranes, as well as the gastrointestinal and genital tract. They are most frequently isolated from the oral cavity and detected in approximately 31% to 55% of healthy individuals. Some yeasts are benign commensals and can switch to a pathogenic state in relation to the health status of the individual and invasiveness of the organism. Among invasion mechanisms, C. albicans and C. dubliniensis are known to change phenotypically from yeast to hyphae; in some species related to candidiasis, genotypic changes include resistance in previously sensitive strains among exposure to antifungal. Geographic factors play an important role in the distribution of infection-related species. Studies on geographic diversity help detect pathogens with high virulence or increased incidence and prevalence. It is estimated that the global incidence of candidiasis is between 4-7 cases / 100,000 inhabitants / year. Candidiasis is treated with a limited variety of antifungal drugs; among these, polyenes, such as amphotericin B and nystatin which alter the cell membrane and azoles that inhibit ergosterol synthesis. There are currently few antifungal agents, therefore mycotic infection treatment options are reduced. Oral cavity samples of 115 healthy individuals were analyzed and isolated yeast strains were identified by RFLP-PCR and CHROMagar. Sensitivity tests to Fluconazole and Amphotericin B were performed by the sensi-disc method.

1 - UABC, Cell Biology, Av. De la Claridad sin numero, Mexicali, B.C., 21376, Mexico
2 - UABC, Cell Biology, Av. De la Claridad sin numero, Cell Biology, Mexicali, B.C., 21376, México
3 - UABC, Biochemistry, Av. De la Claridad sin numero, Mexicali, B.C., 21376, México

Keywords:
Candida
Oral cavity
PCR-RFLP
Antifungical.

Presentation Type: Oral Paper:Papers for Sections
Session: 67
Location: Salon 1/The Shaw Conference Centre
Date: Wednesday, July 29th, 2015
Time: 1:45 PM
Number: 67002
Abstract ID:283
Candidate for Awards:None