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Abstract Detail



Host/Plant Pathogen Interactions and Plant Health Management

Xiang, Yu [1], Bernardy, Mike [1], Bhagwat, Basdeo [1], Su, li [1], Li, Zhengnan [1], Wiersma, Paul [1], You, Frank M. [2].

Detection andcharacterization of viruses associated with sweet cherry mottling leaf diseases.

Virus diseases in fruit trees have an extended impact than viruses in annual crops because the diseases cause cumulative loss over the years and the infected trees need to be eliminated from orchards. Cherry green ring mottle disease (CGRMD), cherry mottle leaf disease (CMLD), cherry necrotic rusty mottle disease (CNRMD), cherry rusty mottle disease (CRMD), and cherry twisted leaf disease (CTLD) are a group of graft-transmissible diseases, resulting in distinct or mixed types of mottling leaf symptoms in sweet cherry trees. The diseases are a major constraint to cherry production in terms of fruit quality and yield as well as a challenge for the development of virus-free propagation materials. Virus species and populations in nine sweet cherry trees that were infected by several cherry mottling leaf diseases were analyzed using deep-sequencing and reverse transcription PCR. The major virus species identified in the samples are Cherry mottle leaf virus (CMLV), Apple chlorotic leaf spot virus (ACLSV), Cherry green ring mottle (CGRMV), Cherry necrotic rusty mottle virus (CNRMV), Cherry rusty mottle associated virus (CRaMV), Cherry twisted leaf associated virus (CTLaV), Cherry virus A (CVA), Prune dwarf virus (PDV) and Prunus necrotic ringspot virus (PNRSV). The variants of the viruses in the samples are also analyzed. The data provide us a more broad understanding of virus biodiversity in cherry trees, and the correlation between host plant symptoms and virus types. Our results also suggest that mixed virus infection is a common phenomenon in orchards, and strategies for management of the co-infections of fruit tree viruses are needed for control of fruit tree virus diseases in agriculture.


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1 - Agriculture and Agri-Food Canada, Pacific Agri-Food Research Centre, 4200 Highway 97, Summerland, BC, V0H 1Z0, Canada
2 - Agriculture and Agri-Food Canada, Cereal research Centre, 101 Route 100, Morden, MB, R6M 1Y5, Canada

Keywords:
fruit tree virus
sweet cherry mottling leaf diseases
Metagenomics.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PPA038
Abstract ID:351
Candidate for Awards:None


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