Abstract Detail
Biotic and abiotic stress Irani, Solmaz [1], Todd, Christopher D. [1]. Ureide Metabolism Following Drought Stress in Arabidopsis. Ureides are nitrogenous compounds derived from purine catabolism which contribute to nitrogen recycling in plants. Ureides also function in the transport of fixed nitrogen in the nodules of some nitrogen-fixing legumes. Accumulation of these compounds has been reported in a number of legume and non-legume plants under abiotic stresses, suggesting the involvement of ureides in plants’ response or adaptation to stress conditions. We examined ureide accumulation and expression of ureide metabolic genes in Arabidopsis plants subjected to drought stress. The ureide compounds uric acid, allantoin and allantoic acid were quantified using High Performance Liquid Chromatography at multiple time points following withholding water. Expression patterns of ureide metabolic genes were determined by RT-PCR. Ureides accumulated in leaf tissue under drought while transcript levels of several genes coding for enzymes involved in ureide synthesis increased. Moreover, T-DNA mutants which accumulate ureides demonstrated enhanced tolerance to the drought treatment and had fewer reactive oxygen species compared to the Col-0 controls. Our results suggest that ureides and ureide metabolism may play a functional role in mediating the plant drought stress response in Arabidopsis and other species.
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1 - University of Saskatchewan, Biology, 112 Science Place, W.P.Thompson Bld, Saskatoon, SK, S7N 5E2, Canada
Keywords:
Ureides
drought
Arabidopsis
Stress response.
Presentation Type: Oral Paper:Papers for Topics
Session: 7
Location: Salon 13/14/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 8:15 AM
Number: 7002
Abstract ID:392
Candidate for Awards:None | 
