Abstract Detail

Horticulture

Ghosh, Amrita [1], Goyali, Juran C. [1], Igamberdiev, Abir U. [1], Debnath, Samir C. [2].

Detection of DNA methylation pattern in in vitro-derived blueberry (Vaccinium spp.) callus using methylation-sensitive amplification polymorphism (MSAP).

With the advent of plant tissue culture techniques it is extensively employed in blueberries (Vaccinium spp. L., family: Ericaceae), to multiply plants rapidly which provides year around production. Tissue culture regenerants are expected to be same as the donor plant. However, this is not always the same. In the in vitro system during the callus formation plant cells undergoes dedifferentiation which prevalently controlled by chromatin remodelling. Eventually, after the callus formation cells starts regenerating and proliferating through the process of redifferentiation. In the regenerating callus redifferentiation stimulated with the introduction of differential effects of plant growth regulators in the culture medium. Due to the changes in the tissue culture microenvironment plant cells go through the additional stress, this induces genetic and epigenetic instabilities in the regenerants genome. These heritable but potentially reversible changes lead to somaclonal variation. Somaclonal variation includes molecular and phenotypic changes persuade in the in vitro culture due to continuous subculturing and tissue culture derived stress. In vitro-derived stress results in histone modification, chromatin remodelling, activation of transposable elements and altered DNA methylation pattern. Reportedly, variation in DNA methylation pattern is much more frequent in the plant genome during the tissue culture and leads to discrete phenotypic changes. In eukaryotic system DNA methylation plays an important role in cell differentiation, chromatin remodelling, genomic imprinting and gene expression. DNA methylation also has an important role in regulation of plant development.  In higher animal and plants cytosine residues (CpG) methylated to form 5-methylcytosine (^5mCpG/ ^5mCpXpG). In this present study, we have analysed the genome wide cytosine methylation in one hybrid between half-high (V. corymbosum x V. angustifolium) and three low-bush (V. angustifolium Ait.) blueberries, using methylation-sensitive amplification polymorphism (MSAP) technique and differentially methylated regions (DMRs) have been detected not only within the different genotypes but also in the callus derived from the semi-solid media with different plant growth regulator combinations.  

1 - Memorial University Of Newfoundland, Department Of Biology, 232 Elizabeth Avenue, St. John's, NL, A1C5S7, Canada
2 - Agriculture and Agri-Food Canada, Atlantic Cool Climate Crop Research Centre, 308 Brookfield Road, Bldg 25, St. John's, NL, A1E 0B2, Canada

Keywords:
Blueberry
DNA methylation
in vitro propagation
somaclonal variation
MSAP.

Presentation Type: Oral Paper:Papers for Topics
Session: 23
Location: Salon 19/20/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 4:45 PM
Number: 23005
Abstract ID:402
Candidate for Awards:2015 Graduate Student Best Oral Presentation Award,2015 Macoun-Hill Graduate Student Travel Award