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Abstract Detail

Host/Plant Pathogen Interactions and Plant Health Management

Wang, Xiben [1], McCallum, Brent [1], Fetch, Tom [1], Bakkeren, Guus [2], Saville, Barry [3].

The involvement of reactive oxygen species during the germination and infection of wheat leaf rust fungus, Puccinia triticina. .

Reactive oxygen species (ROS) plays an important role during host-pathogen interactions and it is often an indication of the induced host defense response triggered upon the recognition of the pathogen by the host. In recent years, studies have also suggested that ROS could also contribute the pathogenicity of fungal pathogens. To date, studies on the involvement of ROS in cereal rust diseases have been mainly focused on the importance of oxidative stress response as a defense mechanism in hosts triggered by avirulence P. triticina strains. The potential roles of ROS to the pathogenicity of rust pathogens have not being investigated. In this study, we investigated the production ROS intermediates in Puccinia triticina, an important obligate biotrophic pathogen causing the leaf rust disease on wheat. The presence of different ROS intermediates in various infection related-structures was detected using nitroblue tetrazolium (NBT), 3,3'-diaminobenzidine (DAB) and 2′,7′-dichlorodihydrofluorescein diacetate (H2DCF). The production of ROS in P. triticina was examined both in vitro and in vivo. Our results show that the production of ROS is commonly produced by P. triticina. ROS intermediates can be detected in infection-related structures produced on artificial media and the living plants, including germ tubes, appressoria, substomatal vesicles, intercellular infection hyphae and haustoria. The commonly presence of these ROS intermediates in P. triticina infection-related structures produced in different environments and infection stages suggests that these molecules could be important for the pathogenicity of this biotrophic cereal pathogen.

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1 - Cereal Research Centre, Agriculture and Agri-Food Canada, 101 Route 100, Morden, MB, R6M 1Y5, Canada
2 - Pacific Agri-Food Research Centre, Agriculture and Agri Food Canada, Summerland, BC, VOH 1ZO, Canada
3 - Trent University, Forensic Science Program , Peterborough, ON, K9J 7B8, Canada

leaf rust
reactive oxygen species.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PPA020
Abstract ID:411
Candidate for Awards:None

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