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Abstract Detail



Host/Plant Pathogen Interactions and Plant Health Management

Li, Zhengnan [1], Bhagwat, Basdeo [1], Bernardy, Mike [1], Wu, Yunfeng [2], Xiang, Yu [1].

Molecularcharacterization of a prune dwarf virus isolate and construction of a PDVinfectious cDNA clone.

Prune dwarf virus is a species belonging to the genus Illarvirus, in the family Bromoviridae, and has a tripartite positive-sense single-stranded RNA genome. The virus is one of the most common viruses infecting Prunus spp. worldwide. Complete genome sequences of a new PDV isolate, SP4-5 were determined by deep-sequencing and reverse transcription PCR (RT-PCR). The PDV RNA1 consists of 3375 nucleotides (nt) and contains one open reading frame (ORF) with 3168 nt encoding the virus replicase subunit P1. The RNA2 has 2653 nt with an ORF of 2364 nt encoding replicase subunit P2. The RNA3 is 2294 nt long and encodes the virus movement protein (ORF3, 882 nt) and coat protein (ORF4, 657 nt). Full-length cDNAs of the tripartite RNA genome were then engineered in a plant binary vector between the sequences of a double CaMV 35S promotor and a ribozyme adjacent to a nopaline synthesis terminator. Agro-infiltration of the virus cDNAs in cotyledon leaves of young cucumber plants resulted in symptoms of leaf venial chlorosis and necrosis in the secondary leaves. RT-PCR confirmed the presence of the PDV RNA1, 2 and 3 in the non-inoculated leaves, indicating the ability of the cDNA clones to systemically infect the plants.


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1 - Agriculture and Agri-Food Canada, Pacific Agri-Food Research Centre, 4200 Highway 97, Summerland, BC, V0H 1Z0, Canada
2 - Northwest A & F University, College of Plant Protection, Yangling, Shaanxi, 712100, China

Keywords:
fruit tree virus
infectious cDNA clones.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PPA039
Abstract ID:432
Candidate for Awards:None


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