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Abstract Detail


Roncal, Julissa [1], Guyot, Romain [2], Hamon, Perla [2], Nowak, Michael D. [3], Davis, Aaron [4], DeKochko, Alexandre [5].

Active transposable elements recover species boundaries and geographic structure in Madagascan coffee species.

The completion of the genome assembly for the economically important coffee plant Coffea canephora (Rubiaceae), has allowed the use of bioinformatic tools to identify and characterize a diverse array of transposable elements (TEs) present in this species. An overview of the copy number and location within the C. canephora genome of four TEs is presented. These are tested for their use as molecular markers to unravel the evolutionary history of the C. millotii Complex, a group of six wild coffee species native to Madagascar. Two TEs from the Gypsy superfamily successfully recovered some species boundaries (C. abbayesii, C. richardii, and C. bonnieri) and geographic structure among samples, whereas a TE from the Copia superfamily did not. Notably, species occurring in evergreen moist forests of eastern and southeastern Madagascar were divergent with respect to species in other habitats and regions. Our results suggest that the peak of transpositional activity of the Gypsy and Copia TEs occurred respectively before and after the speciation events of the tested Madagascan species. Estimations of copy number of each TE were not informative to select a TE to unravel the evolutionary history of closely related species.

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1 - Memorial University Of Newfoundland, 232 Elizabeth Avenue, St. John\'s, N/A, A1B 3X9, Canada
2 - Institute de Recherche pour le Developpment, UMR-DIADE, 911 Av. Agropolis, B.P. 64501, Montpellier, 34394 Cedex 5 , France
3 - Stockholm University, Science for Life Laboratory, Tomtebodavägen 23, Solna, 17165, Sweden
4 - Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 4EP, UK
5 - Institute de Recherche pour le Developpment, UMR-DIADE, 911 Av. Agropolis, Montpellier, 34394 Cedex 5, France

LTR retrotransposons
Coffea canephora

Presentation Type: Poster:Posters for Topics
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PPY001
Abstract ID:449
Candidate for Awards:None

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