| Abstract Detail
Systematics Section/ASPT Onuminya, Temitope [1], Ogundipe, Oluwatoyin [1]. Systematic Identification of African Sapindaceae using DNA Barcoding. Sapindaceae Jussieu is a family of flowering plants in the order Sapindales. They exist as trees and shrubs, and tendril-bearing vines with about 140-150 genera and 1400-2000 species worldwide. They are economically, medicinally and aesthetically useful. This research aimed at exploring the diversity of Sapindaceae in western and central africa with particular emphasis on identification of the plant samples as well as generation of DNA barcodes with a view to sharing the DNA barcode sequence in a public database. This was achieved following standard protocols. Extracted DNA samples (119) were deposited at the DNA Bank of the Royal Botanic Gardens, Kew, Richmond and voucher specmens were deposited at the University of Lagos Herbarium, Lagos, Nigeria. Silica gel dried specimens’ yielded good quality DNA unlike the old dried herbarium leaf samples. DNA samples were sent to International Barcode of Life (IBOL) Centre in Guelph, Canada for analysis of the barcode region and sixty-nine (69) DNA barcodes were generated. Barcode data which was hinged on matK and rbcL sequence data have been deposited at the Barcode of Life database (BOLD) website and GenBank for public use. This research can be seen as a basis for further taxonomic research on the family Sapindaceae especially in Africa. Log in to add this item to your schedule
1 - University of Lagos, Department of Botany, Akoka, Yaba, Lagos, 23401, Nigeria
Keywords: Africa Bio-conservation DNA barcodes Molecular identification Sapindaceae.
Presentation Type: Oral Paper:Papers for Sections Session: 19 Location: Salon 9/The Shaw Conference Centre Date: Monday, July 27th, 2015 Time: 2:15 PM Number: 19004 Abstract ID:479 Candidate for Awards:None |