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Abstract Detail

Mycological Section

Cantrell, Sharon A. [1], Godoy-Vitorino, Filipa [2], Perez-Jimenez, Jose R. [3].

Fungal microbiome of two microbial mats in Puerto Rico.

Microbial mats are layered organosedimentary ecosystem, found in a diversity of fresh and saline environments. Daily and seasonal fluctuating physicochemical gradients characterize these ecosystems resulting in microenvironments that harbor specific microbial communities. Since 2004, two Puerto Rican microbial mat formations have been studied using traditional and molecular techniques such as TRFLP and cloning. The objective of the present work was to document for the first time using next-generation sequencing analysis the fungal microbiome of two microbial mats from Puerto Rico. Samples from a young transitional and a continuous mature microbial mat formations were taken in December 2012. The mats were aseptically divided in three layers (top, intermediate and bottom) and DNA was extracted from each layer using MoBio UltraClean soil DNA Kit and underwent 454 pyrosequencing. A total of 236 fungal OTUs were identified.  Phaeoacremonium was the dominant OTU (51%) followed by Hypocrea (15%) and Coniothyrium (8%). Eight percent of the OTUs were unidentifiable. Cluster analysis shows that samples group by mat formation. The layers of the young transitional mat are more similar in their fungal community that the layers of the mature mat. The results support previous studies that demonstrated the differences between mat formations and layers.

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1 - Universidad del Turabo, Biology, PO Box 3030, Gurabo, PR, 00778, USA
2 - Inter American University of Puerto Rico - Metropolitan Campus, Natural Sciences, San Juan, PR, 00919, USA
3 - Universidad del Turabo, Biology, PO Box 3030, Gurabo, PR, 00778, United States


Presentation Type: Poster:Posters for Sections
Session: P
Location: Hall D/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 5:30 PM
Number: PMY048
Abstract ID:481
Candidate for Awards:None

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