| Abstract Detail
Recent advances in phylogeny and systematics of Brassicaceae Schranz, M. Eric [1], Mohammadin, Setareh [2]. Positionally-Conserved but Sequence-Diverged: Identification of Long non-coding RNAs in the Brassicaceae and Cleomaceae. Long non-coding RNAs (LncRNAs) have been identified as gene regulatory elements that influence the transcription of their neighbouring protein-coding genes. The discovery of LncRNAs in animals has stimulated genome-wide scans for these elements across plant genomes. Recently, 6480 LincRNAs were putatively identified in Arabidopsis thaliana (Brassicaceae), however there is limited information on their conservation. Using a phylogenomics approach, we assessed the positional and sequence conservation of these LncRNAs by analyzing the genomes of the basal Brassicaceae species Aethionema arabicum and Tarenaya hassleriana of the sister-family Cleomaceae. Furthermore, we generated transcriptomes for another three Aethionema species and one other Cleomaceae species to validate their transcriptional activity. We show that a subset of LncRNAs are highly diverged at the nucleotide level, but conserved by position (syntenic). Positionally conserved LncRNAs that are expressed neighbour important developmental and physiological genes. Interestingly, >70% of the positionally conserved LncRNAs are located in sub-telomeric regions of the Arabidopsis thaliana chromosomes. These results highlight the importance of analysing the position of non-coding genetic elements in plants, including LncRNAs, when studying their role in gene expression regulation.
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1 - Wageningen University, Biosystematics Group, P.O. Box 16, Wageningen, 6700AP, The Netherlands
2 - Wageningen University, Biosystematics Group, P.O. Box 16, Wageningen, 6700AP, The Netherlands
Keywords:
Brassicaceae
long non-coding RNAs
phylogenomics.
Presentation Type: Colloquium Presentations
Session: C2
Location: Salon 8/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 3:45 PM
Number: C2006
Abstract ID:513
Candidate for Awards:None |
