| Abstract Detail
Mycological Section Nguyen, Hai D.T. [1], McMullin, David [2], Ponomareva, Ekaterina [1], Miller, J. David [2], Seifert, Keith A. [1]. Ochratoxin A production in Penicillium thymicola revealed by mass spectrometry and genomics. Ochratoxin A (OTA) is a mycotoxin produced by some fungi in the genera Aspergillus and Penicillium that grow on various economically important agricultural crops and food products. This toxin is a group 2B carcinogen, nephrotoxic and regulated internationally countries, notably member States of the EU. Penicillium thymicola was not previously reported to produce OTA. However, using quantitative LC-MS/MS, we identified OTA in the liquid culture extract of P. thymicola DAOM 180753 originally isolated from cheddar cheese. Subsequently, the genome of this strain was sequenced, assembled and annotated. Although the biosynthesis of OTA is not fully understood, the compound includes non-ribosomal peptide and polyketide components, and thus polyketide synthase genes (PKS) are involved. Preliminary analysis of the genome uncovered a putative PKS gene almost identical to that of a strain of P. verrucosum, a closely related species to P. thymicola well known for its ability to produce OTA. This is the first report of a regulated mycotoxin produced by P. thymicola and a new record of the species in Canada. Log in to add this item to your schedule
1 - Eastern Cereal and Oilseed Research Centre, Agriculture & Agri-Food Canada, 960 Carling Ave., Ottawa, ON, K1A0C6, Canada 2 - Carleton University, Department of Chemistry, 1125 Colonel By Drive, Ottawa, ON, K1S5B6, Canada
Keywords: Penicillium thymicola Ochratoxin A genome sequencing Mass Spectrometry.
Presentation Type: Oral Paper:Papers for Sections Session: 74 Location: Salon 1/The Shaw Conference Centre Date: Wednesday, July 29th, 2015 Time: 4:30 PM Number: 74004 Abstract ID:535 Candidate for Awards:None |