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Abstract Detail

Biotic and abiotic stress

Jiang, Yunfei [1], Lahlali, Rachid [2], Karunakaran, Chithra [2], Davis, Arthur [3], Bueckert, Rosalind [1].

Seed set, pollen morphology, and pollen surface composition response to heat stress in field pea.

Pea (Pisum sativum L.) is a major legume crop grown in a semi-arid climate in western Canada, where heat stress affects pollination, seed set and yield. Seed set and pod growth characteristics, along with in vitro percentage pollen germination, pollen tube growth, and pollen surface composition, were measured in two pea cultivars (CDC Golden and CDC Sage) subjected to five maximum temperature regimes ranging from 24 to 36oC. Heat stress reduced percentage pollen germination, pollen tube length, pod length, seed number per pod, and the seed-ovule ratio. Percentage pollen germination of CDC Sage was greater than CDC Golden at 36oC. No visible morphological differences in pollen grains or the pollen surface were observed between the heat and control treated pea. However, pollen wall (intine) thickness increased due to heat stress. Mid Infrared Attenuated Total Reflectance (MIR-ATR) spectra revealed that the chemical composition (lipid, proteins, and carbohydrates) of each cultivar’s pollen grains responded differently to heat stress. The lipid region of the pollen coat and exine of CDC Sage was more stable compared to CDC Golden at 36oC. Secondary derivatives of ATR spectra indicated the presence of two lipid types, with different amounts present in pollen grains from each cultivar.

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1 - University of Saskatchewan, Department of Plant Sciences, 51 Campus Drive, Saskatoon, SK, S7N 5A8, Canada
2 - Canadian Light Source, 44 Innovation Boulevard, Saskatoon, SK, S7N 2V3, Canada
3 - University Of Saskatchewan, Department Of Biology, 112 Science Place, Saskatoon, SK, S7N 5E2, Canada

Field pea
seed set
pollen morphology
pollen surface composition.

Presentation Type: Oral Paper:Papers for Topics
Session: 7
Location: Salon 13/14/The Shaw Conference Centre
Date: Monday, July 27th, 2015
Time: 10:15 AM
Number: 7009
Abstract ID:541
Candidate for Awards:CSA Graduate Student Competition for Best Paper Presentation

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